Department of Biology, McGill University, Montreal, Quebec, Canada H2X 3H5.
Mech Ageing Dev. 2011 Oct;132(10):519-21. doi: 10.1016/j.mad.2011.08.006. Epub 2011 Aug 27.
The nematode worm Caenorhabditis elegans has been used to identify hundreds of genes that influence longevity and thereby demonstrate the strong influence of genetics on lifespan determination. In order to simplify lifespan studies in worms, many researchers have employed 5-fluoro-2'-deoxyuridine (FUdR) to inhibit the development of progeny. While FUdR has little impact on the lifespan of wild-type worms, we demonstrate that FUdR causes a dramatic, dose-dependent, twofold increase in the lifespan of the mitochondrial mutant gas-1. Thus, the concentration of FUdR employed in a lifespan study can determine whether a particular strain is long-lived or short-lived compared to wild-type.
秀丽隐杆线虫已被用于鉴定数百个影响寿命的基因，从而证明了遗传对寿命决定的强大影响。为了简化线虫的寿命研究，许多研究人员使用 5-氟-2'-脱氧尿苷 (FUdR) 来抑制后代的发育。虽然 FUdR 对线虫的寿命几乎没有影响，但我们证明 FUdR 会导致线粒体突变体 gas-1 的寿命显著、剂量依赖性地增加两倍。因此，寿命研究中使用的 FUdR 浓度可以决定与野生型相比，特定菌株是长寿还是短命。
