Tamez González Aura A, AlOkda Abdelrahman, Jacob-Tomas Suleima, Van Raamsdonk Jeremy M
McGill University, Montreal, Quebec, Canada.
Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
MicroPubl Biol. 2024 Sep 4;2024. doi: 10.17912/micropub.biology.001297. eCollection 2024.
has been used extensively for research on the biology of aging due to its genetic tractability and short lifespan. In order to measure lifespan, populations of worms are synchronized so that all of the worms being measured begin the assay at the same age. This is typically accomplished by simply picking worms of a particular developmental stage to start the lifespan experiment or through bleaching, a process through which the body of the worm is dissolved in a solution of bleach (sodium hypochlorite) and sodium hydroxide to release its fertilized eggs. In this experiment, we examined the effect of bleaching on lifespan in wild-type worms and the long-lived mitochondrial mutant . We found that bleaching did not affect the longevity of wild-type worms or mutants. While we cannot exclude the possibility that bleaching affects the lifespan of specific genetic mutants, our results indicate that wild-type longevity is unaffected and that for at least some genetic mutants bleaching can be used for synchronization prior to initiating a lifespan experiment.
由于其遗传易处理性和短寿命，它已被广泛用于衰老生物学的研究。为了测量寿命，线虫群体被同步化，以便所有被测量的线虫在相同年龄开始实验。这通常通过简单地挑选特定发育阶段的线虫来开始寿命实验来完成，或者通过漂白来实现，漂白是一个将线虫身体溶解在漂白剂（次氯酸钠）和氢氧化钠溶液中以释放其受精卵的过程。在这个实验中，我们研究了漂白对线虫野生型和长寿线粒体突变体寿命的影响。我们发现漂白不影响野生型线虫或突变体的寿命。虽然我们不能排除漂白影响特定基因突变体寿命的可能性，但我们的结果表明野生型寿命不受影响，并且对于至少一些基因突变体，漂白可用于在开始寿命实验之前进行同步化。
