Department of Cardiac Surgery, the 1st Affiliated Hospital of Sun Yat-Sen University, Guangzhou, PR China.
Eur J Cardiothorac Surg. 2012 Jan;41(1):162-6. doi: 10.1016/j.ejcts.2011.04.031.
The aim of the present study was to observe the changes of hemodynamics, stereology in pulmonary vascular remodeling and messenger RNA (mRNA) expressions of transforming growth factor beta 1, and receptors in carotid artery-jugular vein (CA-JV) shunt pulmonary artery hypertension model of rats.
Thirty-six Sprague-Dawley rats were randomized into three groups: CA-JV group, monocrotaline (MCT) administration group, and control group. Left CA-JV shunts were established in CA-JV group. Dorsal subcutaneous injections of MCT (60 mg kg(-1)) were received in MCT group. Ligations of left common carotid artery and external jugular vein were performed in control group. Right ventricular systolic pressure (RVSP) measurement, histological evaluation of the pulmonary tissue, and mRNA levels of transforming growth factor beta 1 (TGFß1), receptor 1 and receptor 2, were investigated after 6 weeks on MCT group, and after 12 weeks on both control and CA-JV groups.
Compared with control group, RVSP, percentage of fibrous tissue (F%) in pulmonary arterioles, mRNA levels of TGFß1, and receptors of CA-JVand MCT groups increased significantly. Severe hemodynamics change was found in MCT groups. On the other hand, CA-JV group demonstrated more obvious fibrogenesis and TGFß1 signals' upregulation in two pulmonary artery hypertension (PAH) models.
CA-JV shunt model of rats was a well-established PAH animal model simulating congenital heart disease with systemic-pulmonary shunt.
本研究旨在观察颈动脉-颈静脉（CA-JV）分流肺动脉高压大鼠模型血流动力学、肺血管重构体视学和转化生长因子β 1（TGFβ1）及其受体信使 RNA（mRNA）表达的变化。
36 只 Sprague-Dawley 大鼠随机分为 CA-JV 组、野百合碱（MCT）组和对照组。CA-JV 组建立左 CA-JV 分流，MCT 组给予 MCT（60mg/kg）背部皮下注射，对照组行左颈总动脉和颈外静脉结扎。MCT 组 6 周后、对照组和 CA-JV 组 12 周后分别测量右心室收缩压（RVSP）、肺组织学评价和 TGFβ1、受体 1 和受体 2mRNA 水平。
与对照组相比，CA-JV 组和 MCT 组 RVSP、肺小动脉中纤维组织（F%）百分比、TGFβ1 和受体的 mRNA 水平明显升高。MCT 组血流动力学变化明显，而 CA-JV 组两种肺动脉高压（PAH）模型均显示出更明显的纤维化和 TGFβ1 信号上调。
CA-JV 分流大鼠模型是一种模拟先天性心脏病合并体肺分流的成熟 PAH 动物模型。
