Wu Ji, Luo Xiaoju, Huang Yuanyuan, He Yun, Li Zhixian
Department of Ultrasonic Medicine, the First Hospital Affiliated to Guangxi Medical University, Nanning 530021, China
Department of Ultrasonic Medicine, the First Hospital Affiliated to Guangxi Medical University, Nanning 530021, China.
Exp Biol Med (Maywood). 2015 Oct;240(10):1362-72. doi: 10.1177/1535370214566561. Epub 2015 Jan 16.
The continuous changes in pulmonary hemodynamic properties and right ventricular (RV) function in pulmonary arterial hypertension (PAH) have not been fully characterized in large animal model of PAH induced by a carotid artery-jugular vein shunt. A minipig model of PAH was induced by a surgical anastomosis between the left common carotid artery and the left jugular vein. The model was validated by catheter examination and pathologic analyses, and the hemodynamic features and right-ventricle functional characteristics of the model were continuously observed by Doppler echocardiography. Of the 45 minipigs who received the surgery, 27 survived and were validated as models of PAH, reflected by mean pulmonary artery pressure ≥25 mmHg, and typical pathologic changes of pulmonary arterial remodeling and RV fibrosis. Non-invasive indices of pulmonary hemodynamics (pulmonary artery accelerating time and its ratio to RV ventricular ejection time) were temporarily increased, then reduced later, similar to changes in tricuspid annular displacement. The Tei index of the RV was elevated, indicating a progressive impairment in RV function. Surgical anastomosis between carotid artery and jugular vein in a minipig is effective to establish PAH, and non-invasive hemodynamic and right-ventricle functional indices measured by Doppler echocardiography may be used as early indicators of PAH.
在颈动脉-颈静脉分流诱导的肺动脉高压（PAH）大型动物模型中，PAH时肺血流动力学特性和右心室（RV）功能的持续变化尚未得到充分表征。通过将左颈总动脉与左颈静脉进行手术吻合，建立了小型猪PAH模型。通过导管检查和病理分析对模型进行验证，并通过多普勒超声心动图持续观察模型的血流动力学特征和右心室功能特征。在接受手术的45只小型猪中，27只存活并被验证为PAH模型，表现为平均肺动脉压≥25 mmHg，以及肺动脉重塑和RV纤维化的典型病理变化。肺血流动力学的无创指标（肺动脉加速时间及其与RV心室射血时间的比值）先暂时升高，随后降低，类似于三尖瓣环位移的变化。RV的Tei指数升高，表明RV功能逐渐受损。小型猪颈动脉与颈静脉的手术吻合可有效建立PAH，通过多普勒超声心动图测量的无创血流动力学和右心室功能指标可作为PAH的早期指标。
