Sotero-Caio C G, de Souza M J, Cabral-de-Mello D C, Brasileiro-Vidal A C, Guerra M
Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil.
Cytogenet Genome Res. 2011;135(2):111-7. doi: 10.1159/000330918. Epub 2011 Aug 30.
Phosphorylation of serine 10 in histone H3 (H3S10ph) has been extensively analyzed and appears to be a conserved chromatin change associated with chromosome condensation in different eukaryotic organisms. In this work, we report the distribution of H3S10ph during meiosis in monocentric and holokinetic chromosomes of 6 insect species and in mitotic chromosomes of 7 mammalian species, aiming to investigate the labeling patterns in phylogenetically distant groups. The results indicated a very similar phosphorylation timing and distribution pattern among insects. The sex chromosomes of insects analyzed were always undercondensed and hypophosphorylated. Similarly, the micro chromosomes of the bug Pachylis aff pharaonis were also undercondensed and hypophosphorylated. Holokinetic chromosomes of bugs and monocentric chromosomes of grasshoppers and beetles displayed identical phosphorylation pattern in spite of the difference in the centromere type. Among mammals, a uniform chromosome phosphorylation was observed in marsupials, whereas bat chromosomes displayed a longitudinal banding pattern. These data indicate that, in general, the intensity of H3S10 phosphorylation in animal chromosomes is variable among the distinct chromosome types and associated with the degree of chromatin condensation at metaphase, but it may vary between different groups of animals.
组蛋白H3丝氨酸10位点的磷酸化（H3S10ph）已得到广泛分析，并且似乎是一种保守的染色质变化，与不同真核生物中的染色体凝聚有关。在本研究中，我们报告了H3S10ph在6种昆虫的单着丝粒和全动粒染色体减数分裂过程中以及7种哺乳动物有丝分裂染色体中的分布情况，旨在研究系统发育关系较远的类群中的标记模式。结果表明，昆虫之间的磷酸化时间和分布模式非常相似。所分析昆虫的性染色体总是凝聚不足且磷酸化程度较低。同样，法老厚角蝽的微小染色体也凝聚不足且磷酸化程度较低。尽管着丝粒类型不同，但蝽的全动粒染色体以及蝗虫和甲虫的单着丝粒染色体显示出相同的磷酸化模式。在哺乳动物中，有袋类动物的染色体呈现出均匀的磷酸化，而蝙蝠的染色体则呈现出纵向带纹模式。这些数据表明，一般来说，动物染色体中H3S10磷酸化的强度在不同染色体类型之间存在差异，并且与中期染色质凝聚程度相关，但在不同动物类群之间可能有所不同。
