Ambulatorio di Allergologia, Clinica San Carlo, Via Ospedale 21, Paderno Dugnano, Italy. r.asero @ libero.it
Int Arch Allergy Immunol. 2012;157(1):3-10. doi: 10.1159/000324470. Epub 2011 Sep 5.
Shrimp is a frequent cause of food allergy worldwide. Besides tropomyosin, several allergens have been described recently.
We investigated which allergens are involved in Italian shrimp-allergic adults.
Sera from 116 shrimp-allergic patients selected in 14 Italian allergy centers were studied. Skin prick tests with house dust mite (HDM) as well as measurements of IgE to Pen a 1 (shrimp tropomyosin) and whole shrimp extract were performed. All sera underwent shrimp immunoblot analysis, and inhibition experiments using HDM extract as inhibitor were carried out on some Pen a 1-negative sera.
Immunoblots showed much variability. IgE reactivity at about 30 kDa (tropomyosin) was found in <50% of cases, and reactivity at about 67 kDa and >90 kDa was frequent. Further reactivities at 14-18, 25, 43-50, about 60 and about 80 kDa were detected. Most subjects had a history of shrimp-induced systemic symptoms irrespective of the relevant allergen protein. IgE to Pen a 1 were detected in sera from 46 (41%) patients. Skin reactivity to HDM was found in 43/61 (70%) Pen 1-negative subjects and inhibition studies showed that pre-adsorption of sera with HDM extract induced a marked weakening of the signal at >67 kDa.
Several allergens other than tropomyosin are involved in shrimp allergy in adult Italian patients. Some hitherto not described high molecular weight allergens seem particularly relevant in this population and their cross-reactivity with HDM allergens makes them novel potential panallergens of invertebrates.
虾是全世界范围内引起食物过敏的常见原因。除了肌球蛋白外，最近还描述了几种过敏原。
我们研究了意大利虾过敏成人患者中涉及的过敏原。
研究了在 14 个意大利过敏中心选择的 116 例虾过敏患者的血清。进行了屋尘螨（HDM）皮肤点刺试验以及对 Pen a 1（虾肌球蛋白）和全虾提取物的 IgE 测量。所有血清均进行了虾免疫印迹分析，并对一些 Pen a 1 阴性血清进行了使用 HDM 提取物作为抑制剂的抑制实验。
免疫印迹显示出很大的可变性。<50%的病例中发现了约 30 kDa（肌球蛋白）的 IgE 反应性，而约 67 kDa 和>90 kDa 的反应性很常见。还检测到进一步的约 14-18、25、43-50、约 60 和约 80 kDa 的反应性。大多数患者有虾引起的全身性症状史，而与相关过敏原蛋白无关。在 46 例（41%）患者的血清中检测到对 Pen a 1 的 IgE。在 43/61（70%）Pen 1 阴性的患者中发现了对 HDM 的皮肤反应，抑制研究表明，用 HDM 提取物预吸附血清可显著减弱>67 kDa 的信号。
除了肌球蛋白之外，几种过敏原参与了意大利成人虾过敏患者的过敏反应。一些迄今为止尚未描述的高分子量过敏原似乎在该人群中特别相关，它们与 HDM 过敏原的交叉反应性使它们成为无脊椎动物的新型潜在泛过敏原。
