Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
Oncol Rep. 2011 Dec;26(6):1385-92. doi: 10.3892/or.2011.1450. Epub 2011 Sep 5.
The expression patterns of PDCD4, a tumor suppressor, and β-catenin were immunohistologically investigated in gastric carcinoma tissues. In normal gastric tissues, PDCD4 was strongly expressed in the cell nuclei, but weakly expressed in the cytoplasm. In gastric adenocarcinoma tissues, nuclear PDCD4 expression was decreased, while cytoplasmic PDCD4 expression was unchanged or somewhat increased. In gastric signet ring cell carcinoma tissues, PDCD4 expression patterns were different from the expression patterns of the adenocarcinoma tissues, and PDCD4 was localized in the nuclei of the carcinoma cells as a belt in the middle of the epithelial layer. The nuclear localization of PDCD4 in the adenocarcinoma tissues was correlated with the membrane localization of β-catenin, the activation of which stimulates invasion of colon cancer cells. PDCD4 expression was correlated with β-catenin expression in gastric carcinoma cell lines, but not with E-cadherin, as the binding partner in the cell membrane.
我们通过免疫组织化学方法研究了肿瘤抑制因子 PDCD4 和β-catenin 在胃癌组织中的表达模式。在正常胃组织中，PDCD4 强烈表达于细胞核，而在细胞质中表达较弱。在胃腺癌组织中，核 PDCD4 表达减少，而细胞质 PDCD4 表达不变或略有增加。在胃印戒细胞癌组织中，PDCD4 的表达模式与腺癌组织的表达模式不同，PDCD4 定位于上皮层中间的癌细胞核内，呈带状。腺癌组织中 PDCD4 的核定位与β-catenin 的膜定位相关，β-catenin 的激活刺激结肠癌细胞的侵袭。PDCD4 表达与胃癌细胞系中的β-catenin 表达相关，但与细胞膜上的结合伴侣 E-cadherin 无关。
