College of Chemistry, Sichuan University, Chengdu 610064, People's Republic of China.
J Agric Food Chem. 2011 Oct 26;59(20):10839-47. doi: 10.1021/jf2023325. Epub 2011 Sep 28.
In this paper, a novel application of alternating penalty trilinear decomposition (APTLD) for high-performance liquid chromatography with fluorescence detection (HPLC-FLD) has been developed to simultaneously determine the contents of free amino acids in tea. Although the spectra of amino acid derivatives were similar and a large number of water-soluble compounds are coextracted, APTLD could predict the accurate concentrations together with reasonable resolution of chromatographic and spectral profiles for the amino acids of interest owing to its "second-order advantage". An additional advantage of the proposed method is lower cost than traditional methods. The results indicate that it is an attractive alternative strategy for the routine resolution and quantification of amino acids in the presence of unknown interferences or when complete separation is not easily achieved.
本文提出了一种交替惩罚三线性分解（APTLD）在高效液相色谱-荧光检测（HPLC-FLD）中的新应用，用于同时测定茶中游离氨基酸的含量。尽管氨基酸衍生物的光谱相似，并且大量水溶性化合物被共提取，但由于 APTLD 具有“二阶优势”，可以预测感兴趣氨基酸的准确浓度，并合理地分辨色谱和光谱轮廓。该方法的另一个优点是成本低于传统方法。结果表明，在存在未知干扰或难以完全分离的情况下，该方法是一种有吸引力的常规分离和定量氨基酸的替代策略。
