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对游离氨基酸、总酚类、抗氧化活性和嘌呤生物碱进行研究,以评估非山茶属茶叶的健康特性。

Investigation of free amino acid, total phenolics, antioxidant activity and purine alkaloids to assess the health properties of non-Camellia tea.

作者信息

Bi Wu, He Chunnian, Ma Yunyun, Shen Jie, Zhang Linghua Harris, Peng Yong, Xiao Peigen

机构信息

Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China; Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.

PhytoMedix Co., Whippany, NJ 07981, USA.

出版信息

Acta Pharm Sin B. 2016 Mar;6(2):170-81. doi: 10.1016/j.apsb.2015.11.003. Epub 2015 Dec 24.

DOI:10.1016/j.apsb.2015.11.003
PMID:27006902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4788713/
Abstract

To find novel functional beverages from folk teas, 33 species of frequently used non-Camellia tea (plants other than Camellia) were collected and compared with Camellia tea (green tea, pu-erh tea and black tea) for the first time. Data are reported here on the quantities of 20 free amino acids (FAAs) and three purine alkaloids (measured by UHPLC), total polyphenols (measured by Folin-Ciocalteu assay), and antioxidant activity (DPPH). The total amounts of FAAs in non-Camellia tea (0.62-18.99 mg/g) are generally less than that of Camellia tea (16.55-24.99 mg/g). However, for certain FAAs, the quantities were much higher in some non-Camellia teas, such as γ-aminobutyric acid in teas from Ampelopsis grossedentata, Isodon serra and Hibiscus sabdariffa. Interestingly, theanine was detected in tea from Potentilla fruticosa (1.16±0.81 mg/g). Furthermore, the content of polyphenols in teas from A. grossedentata, Acer tataricum subsp. ginnala are significantly higher than those from Camellia tea; teas from I. serra, Pistacia chinensis and A. tataricum subsp. ginnala have remarkable antioxidant activities similar to the activities from green tea (44.23 μg/mL). Purine alkaloids (caffeine, theobromine and theophylline) were not detected in non-Camellia teas. The investigation suggest some non-Camellia teas may be great functional natural products with potential for prevention of chronic diseases and aging, by providing with abundant polyphenols, antioxidants and specific FAAs.

摘要

为了从民间茶饮中寻找新型功能性饮料,首次收集了33种常用的非山茶科茶(除山茶属植物以外的植物),并与山茶科茶(绿茶、普洱茶和红茶)进行比较。本文报道了20种游离氨基酸(FAA)和三种嘌呤生物碱(采用超高效液相色谱法测定)的含量、总多酚(采用福林-酚试剂法测定)以及抗氧化活性(DPPH)。非山茶科茶中游离氨基酸的总量(0.62 - 18.99毫克/克)一般低于山茶科茶(16.55 - 24.99毫克/克)。然而,对于某些游离氨基酸,在一些非山茶科茶中含量要高得多,比如显齿蛇葡萄、溪黄草和玫瑰茄茶中的γ-氨基丁酸。有趣的是,在金露梅茶中检测到了茶氨酸(1.16±0.81毫克/克)。此外,显齿蛇葡萄、茶条槭亚种茶中的多酚含量显著高于山茶科茶;溪黄草、黄连木和茶条槭亚种茶具有与绿茶(44.23微克/毫升)相当的显著抗氧化活性。在非山茶科茶中未检测到嘌呤生物碱(咖啡因、可可碱和茶碱)。该研究表明,一些非山茶科茶可能是具有预防慢性病和衰老潜力的优质功能性天然产物,因为它们富含多酚、抗氧化剂和特定的游离氨基酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/4c2cac23eb72/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/720be7439bf5/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/effe85604f56/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/baa863e24e0d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/3cf736ddfde6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/18ebddf295b8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/e643726afa58/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/4c2cac23eb72/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/720be7439bf5/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/effe85604f56/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/baa863e24e0d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/3cf736ddfde6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/18ebddf295b8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/e643726afa58/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/763f/4788713/4c2cac23eb72/gr6.jpg

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