Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
Anal Chem. 2011 Oct 1;83(19):7283-9. doi: 10.1021/ac201602s. Epub 2011 Sep 6.
We report the detection of a group of endogenous low molecular weight metabolites (LMWM) in mouse brain (80-500 Da) using TiO(2) nanoparticles (NPs) in nanoparticle-assisted laser desorption/ionization-imaging mass spectrometry (Nano-PALDI-IMS) without any washing and separation step prior to MS analysis. The identification of metabolites using TiO(2) NPs was compared with a conventional organic matrix 2,5-dihydroxybenzoic acid (DHB) where signals of 179 molecules were specific to TiO(2) NPs, 4 were specific to DHB, and 21 were common to both TiO(2) NPs and DHB. The use of TiO(2) NPs enabled the detection of a higher number of LMWM as compared to DHB and gold NPs as a matrix. This approach is a simple, inexpensive, washing, and separation free for imaging and identification of LMWM in mouse brain. We believe that the biochemical information from distinct regions of the brain using a Nano-PALDI-IMS will be helpful in elucidating the imbalances linked with diseases in biomedical samples.
我们报告了使用 TiO2 纳米粒子（NPs）在纳米颗粒辅助激光解吸/电离成像质谱（Nano-PALDI-IMS）中检测到一组内源性低分子量代谢物（LMWM）在小鼠大脑中（80-500 Da），而无需在 MS 分析之前进行任何洗涤和分离步骤。使用 TiO2 NPs 对代谢物的鉴定与传统有机基质 2,5-二羟基苯甲酸（DHB）进行了比较，其中 TiO2 NPs 特有的信号为 179 个分子，DHB 特有的信号为 4 个，TiO2 NPs 和 DHB 共有的信号为 21 个。与 DHB 和金 NPs 作为基质相比，TiO2 NPs 的使用能够检测到更多的 LMWM。这种方法简单、廉价，无需洗涤和分离，可用于在小鼠大脑中成像和鉴定 LMWM。我们相信，使用 Nano-PALDI-IMS 从大脑的不同区域获得的生化信息将有助于阐明与生物医学样本中疾病相关的不平衡。
