单细胞纤维共焦背向散射微光谱分析
Fiber confocal back-scattering micro-spectral analysis for single cell.
机构信息
Lab of Medical Optics and Optometry, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
出版信息
Technol Cancer Res Treat. 2011 Oct;10(5):457-63. doi: 10.7785/tcrt.2012.500222.
A fiber confocal back scattering micro-spectrometer (FCBS) was established, which combined fiber confocal microscopy with light scattering spectroscopy (LSS) for early diagnosis of the cancer cell at cellular level. An adherent monolayer human normal gastric epithelium line GES-1 and a carcinoma cell line NCI-N87 as well as a normal liver cell line L02 and a high-metastatic-potential hepatocellular carcinoma cell line HCC-LM3 were measured respectively. The spectral results showed that micro-back-scattering intensity from GES-1 cell and L02 cell possessed interesting oscillations in contrast to NCI-N87 and HCC-LM3 cells. There was significant difference between the spectra of the normal and the cancer cells (p<0.001). This demonstrates that the FCBS system here is able to distinguish dysplastic cells from normal cells at cellular level.
建立了一种光纤共焦背散射微光谱仪（FCBS），将光纤共焦显微镜与光散射光谱学（LSS）相结合，用于在细胞水平上对癌细胞进行早期诊断。分别测量了贴壁单层人正常胃上皮细胞系 GES-1 和癌细胞系 NCI-N87 以及正常肝细胞系 L02 和高转移潜能肝癌细胞系 HCC-LM3。光谱结果表明，与 NCI-N87 和 HCC-LM3 细胞相比，GES-1 细胞和 L02 细胞的微背散射强度具有有趣的振荡。正常细胞和癌细胞的光谱之间存在显著差异（p<0.001）。这表明这里的 FCBS 系统能够在细胞水平上区分发育不良的细胞和正常细胞。
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