Doussineau Tristan, Bao Cong Yu, Clavier Christian, Dagany Xavier, Kerleroux Michel, Antoine Rodolphe, Dugourd Philippe
Université de Lyon, Lyon, France.
Rev Sci Instrum. 2011 Aug;82(8):084104. doi: 10.1063/1.3628667.
This work presents the implementation of tandem mass spectrometry for experiments on single electrosprayed ions from compounds of megadalton (MDa) molecular weight, using two charge detection devices. The first mass spectrometry stage (first charge detection device) combined with an ion gate allows both mass-to-charge ratio and charge selections of the megadalton ion of interest. The second stage is based on an electrostatic ion trap and consists of an image charge detection tube mounted between two ion mirrors. Single MDa ions can be stored for several dozen milliseconds. During the trapping time, single ions can be irradiated by a continuous wavelength CO(2) laser. We observe stepwise changes in the charge of a single trapped ion owing to multiphoton activation. Illustration of infrared multiphoton dissociation tandem mass spectrometry are given for single megadalton ions of poly(ethylene oxide)s and DNAs.
这项工作展示了串联质谱法在对分子量达兆道尔顿（MDa）的化合物的单电喷雾离子进行实验中的应用，使用了两种电荷检测装置。第一级质谱（第一个电荷检测装置）与一个离子门相结合，可实现对感兴趣的兆道尔顿离子的质荷比和电荷选择。第二级基于一个静电离子阱，由安装在两个离子镜之间的一个镜像电荷检测管组成。单个MDa离子可存储几十毫秒。在捕获期间，单个离子可被连续波长的CO(2)激光照射。我们观察到由于多光子激活，单个捕获离子的电荷发生逐步变化。给出了聚环氧乙烷和DNA的单个兆道尔顿离子的红外多光子解离串联质谱的示例。
