Chemistry Department, Indiana University, 800 E. Kirkwood Avenue, Bloomington, Indiana 47404, United States.
Chem Rev. 2022 Apr 27;122(8):7415-7441. doi: 10.1021/acs.chemrev.1c00377. Epub 2021 Oct 12.
Charge detection mass spectrometry (CDMS) is a single-particle technique where the masses of individual ions are determined from simultaneous measurement of their mass-to-charge ratio (/) and charge. Masses are determined for thousands of individual ions, and then the results are binned to give a mass spectrum. Using this approach, accurate mass distributions can be measured for heterogeneous and high-molecular-weight samples that are usually not amenable to analysis by conventional mass spectrometry. Recent applications include heavily glycosylated proteins, protein complexes, protein aggregates such as amyloid fibers, infectious viruses, gene therapies, vaccines, and vesicles such as exosomes.
电荷检测质谱法（CDMS）是一种单颗粒技术，可通过同时测量单个离子的质荷比（/）和电荷来确定其质量。该技术可测定数千个单个离子的质量，然后对结果进行分类，给出质谱图。采用这种方法，可测量通常不适用于常规质谱分析的异质和高分子量样品的精确质量分布。最近的应用包括高度糖基化蛋白、蛋白复合物、蛋白聚集物（如淀粉样纤维）、传染性病毒、基因疗法、疫苗和囊泡（如外泌体）等。
