Li E, Kornfeld S
J Biol Chem. 1979 Apr 25;254(8):2754-8.
Previous work has shown that vesicular stomatitis virus-infected Chinese hamster ovary cells contain a major high molecular weight lipid-linked oligosaccharide which is transferred en bloc to protein during the formation of the asparagine-linked complex-type oligosaccharides of the vesicular stomatitis virus G protein (Tabas, I., Schlesinger, S., and Kornfeld, S. (1978) J. Biol. Chem. 253, 716-722). We now report the characterization of a second, lower molecular weight lipid-linked oligosaccharide. The oligosaccharide portion of this molecule was isolated and its structure was determined by methylation analysis, digestion with exoglycosidases, acetolysis and Smith periodate degradation to be: (formula: see text). Several lines of evidence are presented which indicate that this lipid-linked oligosaccharide is primarily involved in the assembly of the major lipid-linked oligosaccharide rather than in the direct glycosylation of proteins.
先前的研究表明，感染水泡性口炎病毒的中国仓鼠卵巢细胞含有一种主要的高分子量脂质连接寡糖，在水泡性口炎病毒G蛋白的天冬酰胺连接的复合型寡糖形成过程中，该寡糖会整体转移至蛋白质上（塔巴斯，I.，施莱辛格，S.，和科恩菲尔德，S.（1978年）《生物化学杂志》253卷，716 - 722页）。我们现在报告第二种分子量较低的脂质连接寡糖的特性。分离出该分子的寡糖部分，并通过甲基化分析、外切糖苷酶消化、乙酰解和高碘酸史密斯降解确定其结构为：（分子式：见正文）。提供了几条证据表明，这种脂质连接寡糖主要参与主要脂质连接寡糖的组装，而非蛋白质的直接糖基化。
