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1
Mutant of Chinese hamster ovary cells with altered mannose 6-phosphate receptor activity is unable to synthesize mannosylphosphoryldolichol.具有改变的甘露糖6 - 磷酸受体活性的中国仓鼠卵巢细胞突变体无法合成甘露糖基磷酸多萜醇。
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2296-300. doi: 10.1073/pnas.79.7.2296.
2
A single enzyme catalyzes the synthesis of the mannosylphosphoryl derivative of dolichol and retinol in rat liver and Chinese hamster ovary cells.一种单一的酶催化大鼠肝脏和中国仓鼠卵巢细胞中多萜醇和视黄醇的甘露糖基磷酸化衍生物的合成。
J Biol Chem. 1985 Jan 10;260(1):232-6.
3
A block at Man5GlcNAc2-pyrophosphoryldolichol in intact but not disrupted castanospermine and swainsonine-resistant Chinese hamster ovary cells.在完整但未破坏的栗精胺和苦马豆素抗性中国仓鼠卵巢细胞中，Man5GlcNAc2-焦磷酸化多萜醇存在阻断作用。
J Biol Chem. 1990 Feb 5;265(4):2296-305.
4
Two mutants of Dictyostelium discoideum that lack a sulfated carbohydrate antigenic determinant synthesize a truncated lipid-linked precursor of N-linked oligosaccharides.
J Biol Chem. 1989 Apr 5;264(10):5653-9.
5
A Chinese hamster ovary cell mutant F2A8 utilizes polyprenol rather than dolichol for its lipid-dependent asparagine-linked glycosylation reactions.一只中国仓鼠卵巢细胞突变体F2A8在其脂质依赖性天冬酰胺连接的糖基化反应中利用聚戊烯醇而非多萜醇。
J Biol Chem. 1988 Aug 5;263(22):10774-82.
6
The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.E类Thy-1阴性突变小鼠淋巴瘤细胞中的主要糖基化缺陷是无法合成多萜醇磷酸甘露糖。
J Biol Chem. 1980 May 25;255(10):4441-6.
7
Nonglucosylated oligosaccharides are transferred to protein in MI8-5 Chinese hamster ovary cells.非糖基化寡糖被转移至MI8-5中国仓鼠卵巢细胞中的蛋白质上。
Glycobiology. 1999 Jan;9(1):65-72. doi: 10.1093/glycob/9.1.65.
8
The mannose 6-phosphate receptor of Chinese hamster ovary cells. Compartmentalization of acid hydrolases in mutants with altered receptors.中国仓鼠卵巢细胞的甘露糖6-磷酸受体。受体改变的突变体中酸性水解酶的区室化。
J Biol Chem. 1981 Oct 25;256(20):10623-7.
9
Addition of a mannose-6-phosphate-containing oligosaccharide alters cellular processing of low density lipoprotein by parental and LDL-receptor-defective Chinese hamster ovary cells.
J Cell Sci. 1984 Jun;68:183-94. doi: 10.1242/jcs.68.1.183.
10
A concanavalin A-resistant Chinese hamster ovary cell line is deficient in the synthesis of [3H]glucosyl oligosaccharide-lipid.
J Biol Chem. 1979 Sep 25;254(18):9167-77.

引用本文的文献

1
N-glycome of the Lysosomal Glycocalyx is Altered in Niemann-Pick Type C Disease (NPC) Model Cells.溶酶体糖萼的 N-聚糖在尼曼-匹克 C 型疾病（NPC）模型细胞中发生改变。
Mol Cell Proteomics. 2018 Apr;17(4):631-642. doi: 10.1074/mcp.RA117.000129. Epub 2018 Jan 24.
2
Large induces functional glycans in an O-mannosylation dependent manner and targets GlcNAc terminals on alpha-dystroglycan.大蛋白以 O-岩藻糖基化依赖的方式诱导功能性聚糖，并靶向α- dystroglycan 上的 GlcNAc 末端。
PLoS One. 2011 Feb 9;6(2):e16866. doi: 10.1371/journal.pone.0016866.
3
Analysis and metabolic engineering of lipid-linked oligosaccharides in glycosylation-deficient CHO cells.糖基化缺陷 CHO 细胞中脂连接寡糖的分析与代谢工程。
Biochem Biophys Res Commun. 2010 Apr 23;395(1):36-41. doi: 10.1016/j.bbrc.2010.03.117. Epub 2010 Mar 21.
4
Disruption of mannose activation in Leishmania mexicana: GDP-mannose pyrophosphorylase is required for virulence, but not for viability.墨西哥利什曼原虫中甘露糖激活的破坏：GDP-甘露糖焦磷酸化酶是毒力所必需的，但不是生存能力所必需的。
EMBO J. 2001 Jul 16;20(14):3657-66. doi: 10.1093/emboj/20.14.3657.
5
Dolichol phosphate mannose synthase (DPM1) mutations define congenital disorder of glycosylation Ie (CDG-Ie).磷酸多萜醇甘露糖合成酶（DPM1）突变可导致先天性糖基化障碍Ie型（CDG-Ie）。
J Clin Invest. 2000 Jan;105(2):191-8. doi: 10.1172/JCI7302.
6
DPM2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of DPM1, and binding of dolichol phosphate.DPM2调节哺乳动物细胞中磷酸多萜醇甘露糖的生物合成：DPM1的正确亚细胞定位和稳定，以及磷酸多萜醇的结合。
EMBO J. 1998 Sep 1;17(17):4920-9. doi: 10.1093/emboj/17.17.4920.
7
Protein C-mannosylation is enzyme-catalysed and uses dolichyl-phosphate-mannose as a precursor.蛋白质C-甘露糖基化是由酶催化的，并以磷酸多萜醇甘露糖作为前体。
Mol Biol Cell. 1998 Feb;9(2):291-300. doi: 10.1091/mbc.9.2.291.
8
Human and Saccharomyces cerevisiae dolichol phosphate mannose synthases represent two classes of the enzyme, but both function in Schizosaccharomyces pombe.人和酿酒酵母的磷酸多萜醇甘露糖合酶代表了该酶的两类，但两者在粟酒裂殖酵母中均有功能。
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7873-8. doi: 10.1073/pnas.94.15.7873.
9
Carbohydrate-deficient glycoprotein syndrome: not an N-linked oligosaccharide processing defect, but an abnormality in lipid-linked oligosaccharide biosynthesis?碳水化合物缺乏糖蛋白综合征：并非N-连接寡糖加工缺陷，而是脂连接寡糖生物合成异常？
J Clin Invest. 1994 Nov;94(5):1901-9. doi: 10.1172/JCI117540.
10
Transmembrane movement of a water-soluble analogue of mannosylphosphoryldolichol is mediated by an endoplasmic reticulum protein.甘露糖基磷酸多萜醇的水溶性类似物的跨膜运动由一种内质网蛋白介导。
J Cell Biol. 1995 Aug;130(3):529-36. doi: 10.1083/jcb.130.3.529.

本文引用的文献

1
Structural studies of phosphorylated high mannose-type oligosaccharides.磷酸化高甘露糖型寡糖的结构研究
J Biol Chem. 1980 Nov 25;255(22):10847-58.
2
Biosynthetic intermediates of beta-glucuronidase contain high mannose oligosaccharides with blocked phosphate residues.β-葡萄糖醛酸酶的生物合成中间体含有带有封闭磷酸残基的高甘露糖寡糖。
J Biol Chem. 1980 Jul 25;255(14):6633-9.
3
The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.E类Thy-1阴性突变小鼠淋巴瘤细胞中的主要糖基化缺陷是无法合成多萜醇磷酸甘露糖。
J Biol Chem. 1980 May 25;255(10):4441-6.
4
Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling.氯喹通过损害受体再循环抑制溶酶体酶的胞饮作用并增强溶酶体酶的分泌。
J Cell Biol. 1980 Jun;85(3):839-52. doi: 10.1083/jcb.85.3.839.
5
Biosynthesis of lysosomal enzymes in fibroblasts. Phosphorylation of mannose residues.成纤维细胞中溶酶体酶的生物合成。甘露糖残基的磷酸化。
J Biol Chem. 1980 May 25;255(10):4946-50.
6
Biosynthesis of lysosomal enzymes in fibroblasts. Synthesis as precursors of higher molecular weight.成纤维细胞中溶酶体酶的生物合成。以高分子量前体形式合成。
J Biol Chem. 1980 May 25;255(10):4937-45.
7
Beta-glucuronidase binding to human fibroblast membrane receptors.β-葡萄糖醛酸酶与人成纤维细胞膜受体的结合
J Biol Chem. 1980 Jun 10;255(11):5069-74.
8
Defective phosphorylation and processing of beta-hexosaminidase by intact cultured fibroblasts from patients with mucolipidosis III.黏脂贮积症III型患者完整培养成纤维细胞中β-己糖胺酶的磷酸化和加工缺陷
Arch Biochem Biophys. 1982 Jan;213(1):251-7. doi: 10.1016/0003-9861(82)90459-3.
9
Enzymatic phosphorylation of lysosomal enzymes in the presence of UDP-N-acetylglucosamine. Absence of the activity in I-cell fibroblasts.在UDP-N-乙酰葡糖胺存在的情况下溶酶体酶的酶促磷酸化。I型细胞成纤维细胞中缺乏该活性。
Biochem Biophys Res Commun. 1981 Feb 12;98(3):761-7. doi: 10.1016/0006-291x(81)91177-3.
10
Characterization of a membrane-associated receptor from bovine liver that binds phosphomannosyl residues of bovine testicular beta-galactosidase.牛肝中一种与膜相关的受体的特性研究，该受体可结合牛睾丸β-半乳糖苷酶的磷酸甘露糖残基。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4289-93. doi: 10.1073/pnas.78.7.4289.

具有改变的甘露糖6 - 磷酸受体活性的中国仓鼠卵巢细胞突变体无法合成甘露糖基磷酸多萜醇。

Mutant of Chinese hamster ovary cells with altered mannose 6-phosphate receptor activity is unable to synthesize mannosylphosphoryldolichol.

作者信息

Stoll J, Robbins A R, Krag S S

出版信息

Proc Natl Acad Sci U S A. 1982 Apr;79(7):2296-300. doi: 10.1073/pnas.79.7.2296.

DOI:10.1073/pnas.79.7.2296
PMID:6954541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346179/
Abstract

B4-2-1 is a Chinese hamster ovary cell mutant previously isolated and characterized as deficient in mannose 6-phosphate receptor activity. We show here that B4-2-1 is a pleiotropic mutant, defective in biosynthesis of asparagine-linked oligosaccharides, B4-2-1 is unable to synthesize mannosylphosphoryldolichol; the consequences of this defect on glycosylation are (i) biosynthesis of one major lipid-linked oligosaccharide, characterized by its resistance to endoglycosidase H and decreased size; this oligosaccharide is similar to a minor species of lipid-liked oligosaccharide found in parental cells; (ii) transfer of this oligosaccharide to newly synthesized proteins; and (iii) absence of normal "high-mannose" oligosaccharides on mature glycoproteins isolated from B4-2-1; glycoproteins from the mutant contain complex oligosaccharides as well as endoglycosidase H-resistant, alpha-mannosidase-sensitive species. While the glycosylation defect may alter adversely the function of several glycoproteins in the mutant, including that of the mannose 6-phosphate receptor, it appears to have no effect on the formation or function of the mannose 6-phosphate recognition marker on acid hydrolases of B4-2-1.

摘要

B4-2-1是一种先前分离出的中国仓鼠卵巢细胞突变体，其特征是缺乏甘露糖6-磷酸受体活性。我们在此表明，B4-2-1是一种多效性突变体，在天冬酰胺连接的寡糖生物合成方面存在缺陷，B4-2-1无法合成甘露糖基磷酸多萜醇；这种缺陷对糖基化的影响如下：（i）一种主要的脂质连接寡糖的生物合成，其特征是对内切糖苷酶H具有抗性且大小减小；这种寡糖类似于在亲本细胞中发现的一种次要的脂质连接寡糖；（ii）这种寡糖转移到新合成的蛋白质上；（iii）从B4-2-1分离出的成熟糖蛋白上不存在正常的“高甘露糖”寡糖；突变体的糖蛋白含有复杂寡糖以及对内切糖苷酶H有抗性、对α-甘露糖苷酶敏感的种类。虽然糖基化缺陷可能会对突变体中几种糖蛋白的功能产生不利影响，包括甘露糖6-磷酸受体的功能，但它似乎对B4-2-1酸性水解酶上甘露糖6-磷酸识别标记的形成或功能没有影响。