巴西北部和东北部利什曼病血清免疫荧光试验和酶免疫测定的诊断性能指标
Diagnostic performance indices for immunofluorescent tests and enzyme immunoassays of leishmaniasis sera from northern and north-eastern Brazil.
作者信息
Guimarães M C, Celeste B J, Franco E L
机构信息
Department of Preventive Medicine, Faculdade de Medicina da Universidade de São Paulo, Brazil.
出版信息
Bull World Health Organ. 1990;68(1):39-43.
Abstract
A total of 341 sera were screened for anti-Leishmania IgA, IgG, and IgM antibodies by immunofluorescent (IF) tests and enzyme immunoassay (ELISA). Altogether, 292 of the sera originated from patients with clinically as well as parasitologically diagnosed (positive lesion imprint or the Montenegro skin test) cutaneous leishmaniasis; 49 of the sera were from controls from the same base population. In terms of diagnostic performance, the ELISAs for IgG and IgM yielded indices of diagnostic utility, and the positive predictive value for the IgG-ELISA was 94.6%. A remarkably high specificity (100%) was obtained with the IgA-IF test, but its sensitivity was very low.
摘要
通过免疫荧光（IF）试验和酶免疫测定（ELISA）对总共341份血清进行了抗利什曼原虫IgA、IgG和IgM抗体筛查。其中，292份血清来自临床及寄生虫学诊断（病变印记阳性或蒙氏皮肤试验阳性）的皮肤利什曼病患者；49份血清来自同一基础人群的对照者。在诊断性能方面，IgG和IgM的ELISA产生了诊断效用指数，IgG-ELISA的阳性预测值为94.6%。IgA-IF试验获得了非常高的特异性（100%），但其敏感性非常低。
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