通过免疫过氧化物酶测定法进行皮肤利什曼病的血清学诊断。
Cutaneous leishmaniasis serodiagnosis by immunoperoxidase assay.
作者信息
Halevy D, Sarov B, Sarov I, el-On J
机构信息
Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel.
出版信息
Isr J Med Sci. 1990 Sep;26(9):485-90.
Immunoperoxidase assay was used for the determination of serum-specific anti-leishmanial IgG antibodies in 65 patients with cutaneous leishmaniasis (CL), in 5 with visceral leishmaniasis (VL) and in 84 controls. A significant difference was observed between CL and VL sera and the control sera when either Leishmania major, L. donovani or L. aethiopica intact promastigotes were used as antigens. CL patients showed similar activity against L. major and L. donovani antigen (titers 4-64 and less than 2-64 respectively), whereas VL patients showed a higher activity with the homologous antigen L. donovani (titers 128-2,048). A high and significant correlation was demonstrated between immunoperoxidase assay, immunofluorescent assay and radioimmunoassay. No cross-reactivity was found with sera from malaria patients.
采用免疫过氧化物酶测定法检测65例皮肤利什曼病（CL）患者、5例内脏利什曼病（VL）患者及84名对照者血清中的特异性抗利什曼原虫IgG抗体。当分别以硕大利什曼原虫、杜氏利什曼原虫或埃塞俄比亚利什曼原虫的完整前鞭毛体作为抗原时，CL和VL患者血清与对照者血清之间存在显著差异。CL患者对硕大利什曼原虫和杜氏利什曼原虫抗原表现出相似的活性（滴度分别为4 - 64和小于2 - 64），而VL患者对同源抗原杜氏利什曼原虫表现出更高的活性（滴度为128 - 2048）。免疫过氧化物酶测定法、免疫荧光测定法和放射免疫测定法之间显示出高度显著的相关性。未发现与疟疾患者血清有交叉反应。
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