Jiangsu University of Science and Technology, Zhenjiang 212018, China.
J Eukaryot Microbiol. 2011 Nov-Dec;58(6):539-41. doi: 10.1111/j.1550-7408.2011.00579.x. Epub 2011 Sep 7.
By sequencing the entire ribosomal RNA (rRNA) gene region of Nosema heliothidis isolated from cotton bollworm (Helicoverpa armigera), we showed that its gene organization is similar to the type species, Nosema bombycis: the 5'-large subunit rRNA (2,490 bp)-internal transcribed spacer (192 bp)-small subunit rRNA (1,232 bp)-intergenic spacer (274 bp)-5S rRNA (115 bp)-3'. We constructed two phylogenetic trees, analyzed phylogenetic relationships, examined rRNA organization of microsporidia, and compared the secondary structure of small subunit rRNA with closely related microsporidia. The latter two features may provide important information for the classification and phylogenetic analysis of microsporidia.
通过对从棉铃虫（Helicoverpa armigera）中分离出的微孢子虫 Nosema heliothidis 的核糖体 RNA（rRNA）全基因区域进行测序，我们表明其基因组织类似于模式种，Nosema bombycis：5'-大亚基 rRNA（2490 bp）-内部转录间隔区（192 bp）-小亚基 rRNA（1232 bp）-基因间间隔区（274 bp）-5S rRNA（115 bp）-3'。我们构建了两棵系统发育树，分析了系统发育关系，检查了微孢子虫的 rRNA 组织，并与密切相关的微孢子虫进行了小亚基 rRNA 的二级结构比较。后两个特征可能为微孢子虫的分类和系统发育分析提供重要信息。
