Ku Chin-Tai, Wang Chih-Yuan, Tsai Yi-Chun, Tzeng Ching-Chou, Wang Chung-Hsiung
Department of Entomology, National Taiwan University, Taipei 106, Taiwan.
J Invertebr Pathol. 2007 May;95(1):71-6. doi: 10.1016/j.jip.2006.11.008. Epub 2007 Jan 30.
In this study, a new microsporidian, PX2, was isolated from the diamondback moth, Plutella xylostella, and then compared with another isolate (PX1), and with Nosema spodopterae and N. bombycis. Sequence data showed that the rRNA gene organizations of PX1 and PX2 exhibited a typical Nosema-specific organization: 5'-LSUrRNA (large subunit ribosomal RNA)-ITS (internal transcribed spacer)-SSUrRNA-IGS (intergenic spacer)-5S-3'. Phylogenetic analysis (maximum likelihood, neighbor joining, maximum parsimony, and Bayesian analysis) of the LSUrRNA and SSUrRNA gene sequences, and the sequences of the alpha-tubulin, beta-tubulin, and RPB1 (DNA dependent RNA polymerase II largest subunit) genes found that PX1 was closer to N. bombycis and N. spodopterae than to PX2. Comparison of the identities of the rRNA domains and of the other three genes showed a high divergence in the sequences of the rRNA spacer regions (ITS and IGS). This is consistent with the hypothesis that PX2, if not PX1, might represent a new Nosema species.
在本研究中，从小菜蛾（Plutella xylostella）中分离出一种新的微孢子虫PX2，然后将其与另一种分离株（PX1）以及斜纹夜蛾微孢子虫（Nosema spodopterae）和家蚕微孢子虫（N. bombycis）进行比较。序列数据显示，PX1和PX2的rRNA基因组织呈现出典型的微孢子虫特异性组织形式：5'-大亚基核糖体RNA（LSUrRNA）-内部转录间隔区（ITS）-小亚基核糖体RNA（SSUrRNA）-基因间隔区（IGS）-5S-3'。对LSUrRNA和SSUrRNA基因序列以及α-微管蛋白、β-微管蛋白和RPB1（DNA依赖性RNA聚合酶II最大亚基）基因序列进行系统发育分析（最大似然法、邻接法、最大简约法和贝叶斯分析）发现，与PX2相比，PX1与家蚕微孢子虫和斜纹夜蛾微孢子虫的亲缘关系更近。对rRNA结构域和其他三个基因的同一性比较表明，rRNA间隔区（ITS和IGS）的序列存在高度差异。这与以下假设一致，即PX2（如果不是PX1的话）可能代表一种新的微孢子虫物种。
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