评价一种体外细胞测定法，以筛选对斑点叉尾鮰和迟钝爱德华氏菌具有减毒作用的突变体。
Evaluation of an in vitro cell assay to select attenuated bacterial mutants of Aeromonas hydrophila and Edwardsiella tarda to channel catfish.
机构信息
Aquatic Animal Health Research Unit, USDA-ARS, Auburn, AL 36832, USA.
出版信息
J Appl Microbiol. 2011 Dec;111(6):1310-8. doi: 10.1111/j.1365-2672.2011.05146.x. Epub 2011 Sep 20.
AIMS
To evaluate the feasibility of using an in vitro cell assay to select attenuated bacterial mutants.
METHODS AND RESULTS
Using catfish gill cells G1B, the feasibility of using an in vitro assay instead of in vivo virulence assay using live fish to select attenuated bacterial mutants was evaluated in this study. Pearson correlation analysis between in vitro virulence to G1B cells and in vivo virulence of Aeromonas hydrophila and Edwardsiella tarda revealed that there was a significant correlation between the two (r = -0.768, P value = 3.7 × 10(-16)).
CONCLUSIONS
The in vitro cell assay might be initially used to screen large quantities of bacteria to select attenuated mutants of catfish pathogens.
SIGNIFICANCE AND IMPACT OF THE STUDY
The in vitro cell assay using catfish gill cells to identify attenuated mutants of catfish pathogens will reduce cost involved in the in vivo virulence assay that requires many fish and aquariums.
目的
评估使用体外细胞测定法筛选减毒细菌突变体的可行性。
方法和结果
本研究使用鲶鱼鳃细胞 G1B，评估了使用体外测定法代替使用活鱼进行体内毒力测定来筛选减毒细菌突变体的可行性。体外对 G1B 细胞的毒力与嗜水气单胞菌和迟缓爱德华菌体内毒力之间的 Pearson 相关性分析表明，两者之间存在显著相关性（r = -0.768，P 值=3.7×10(-16)）。
结论
体外细胞测定法可初步用于筛选大量细菌，以筛选鲶鱼病原体的减毒突变体。
研究的意义和影响
使用鲶鱼鳃细胞进行的体外细胞测定法来鉴定鲶鱼病原体的减毒突变体将降低体内毒力测定所需的成本，而体内毒力测定需要大量鱼类和水族箱。
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