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两个Sec13p同源物，AtSec13A和AtSec13B，对拟南芥中COPII运输小泡的形成起着冗余作用。

Two Sec13p homologs, AtSec13A and AtSec13B, redundantly contribute to the formation of COPII transport vesicles in Arabidopsis thaliana.

作者信息

Hino Takeshi, Tanaka Yuji, Kawamukai Makoto, Nishimura Kohji, Mano Shoji, Nakagawa Tsuyoshi

机构信息

Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University.

出版信息

Biosci Biotechnol Biochem. 2011;75(9):1848-52. doi: 10.1271/bbb.110331. Epub 2011 Sep 7.

DOI:10.1271/bbb.110331
PMID:21897010
Abstract

COPII vesicles mediate protein transport from ER to Golgi. Sec13 makes up lattice structure with Sec31 to form COPII vesicles. We analyzed expression of two Arabidopsis thaliana Sec13 homologs, AtSec13A and AtSec13B. AtSec13A was expressed in most parts of seedlings, while AtSec13B was partially expressed. Interaction of AtSec13A or AtSec13B with Sec31 homolog was demonstrated by bimolecular fluorescence complementation (BiFC).

摘要

COPII囊泡介导蛋白质从内质网运输到高尔基体。Sec13与Sec31组成晶格结构以形成COPII囊泡。我们分析了两种拟南芥Sec13同源物AtSec13A和AtSec13B的表达情况。AtSec13A在幼苗的大部分部位表达，而AtSec13B则是部分表达。通过双分子荧光互补（BiFC）证明了AtSec13A或AtSec13B与Sec31同源物之间的相互作用。

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