Hua Chengwei, Yi Huaxi, Jiao Lingxia
School of Life Science and Technology, Henan Institute of Science and Technology, China.
Biosci Biotechnol Biochem. 2011;75(9):1807-12. doi: 10.1271/bbb.110354. Epub 2011 Sep 7.
The cDNA encoding β-1,3(4)-glucanase, named PsBg16A, from Paecilomyces sp. FLH30 was cloned, sequenced, and over expressed in Pichia pastoris, with a yield of about 61,754 U mL⁻¹ in a 5-L fermentor. PsBg16A has an open reading frame of 951 bp encoding 316 amino acids, and the deduced amino acid sequence of PsBg16A revealed that it belongs to glycoside hydrolase family 16. The purified recombinant PsBg16A had a pH optimum at 7.0 and a temperature optimum at 70 °C, and randomly hydrolyzed barley β-glucan, lichenin, and laminarin, suggesting that it is a typical endo-1,3(4)-β-glucanase (EC 3.2.1.6) with broad substrate specificity for β-glucans.
从拟青霉属真菌FLH30中克隆、测序并在毕赤酵母中过量表达了编码β-1,3(4)-葡聚糖酶的cDNA，命名为PsBg16A，在5-L发酵罐中的产量约为61,754 U mL⁻¹。PsBg16A具有951 bp的开放阅读框，编码316个氨基酸，推导的PsBg16A氨基酸序列表明它属于糖苷水解酶家族16。纯化的重组PsBg16A的最适pH为7.0，最适温度为70℃，能随机水解大麦β-葡聚糖、地衣多糖和海带多糖，表明它是一种典型的内切-1,3(4)-β-葡聚糖酶（EC 3.2.1.6），对β-葡聚糖具有广泛的底物特异性。
