Chen Kuo-Hsin, Li Ping-Chia, Lin Wei-Hsiang, Chien Chiang-Ting, Low Boon-Hua
Division of General Surgery, Far-Eastern Memorial Hospital, Taipei, Taiwan.
Biosci Biotechnol Biochem. 2011;75(9):1668-76. doi: 10.1271/bbb.110163. Epub 2011 Sep 7.
We determined the effects of a green tea extract with 36% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O₂⁻, H₂O₂ and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O₂⁻, H₂O₂ and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.
我们测定了含36%酒精的绿茶提取物对雌性Wistar大鼠血液酒精含量、氧化应激、脂肪生成、炎症及肝功能的影响。茶酒精通过儿茶素而非咖啡因显著降低了超氧阴离子（O₂⁻）、过氧化氢（H₂O₂）和次氯酸（HOCl）的含量。连续30天灌胃酒精提高了肝脏、胆汁和血液中的活性氧（ROS）水平，增加了4-羟基壬烯醛-蛋白质加合物、肝内库普弗细胞浸润和脂质蓄积，并提高了血浆丙氨酸转氨酶水平。蛋白质免疫印迹分析显示，酒精处理的肝脏中氧化酶（细胞色素P450 2E1（CYP2E1）和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶p47phox蛋白）和脂肪生成酶（固醇调节元件结合蛋白-1c（SREBP-1c）和脂肪酸合酶）的表达降低。茶酒精显著减弱了这些升高的参数。我们得出结论，酒精中的绿茶提取物主要由于儿茶素含量而非咖啡因含量，有效降低了O₂⁻、H₂O₂和HOCl的含量。所研制的茶液通过儿茶素和咖啡因的协同作用减轻了酒精诱导的肝脏氧化损伤和脂肪生成。
