Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China.
Arch Gynecol Obstet. 2012 Mar;285(3):749-55. doi: 10.1007/s00404-011-2049-9. Epub 2011 Sep 7.
Turner syndrome (TS) is associated with deficiency of cellular and humoral immunity. However, the characteristics of lymphocyte subpopulations in Chinese women with TS have not been reported. In this study, the percentage of lymphocyte subpopulations and the mRNA expression of some transcription factors were determined in patients with TS. The effect of the hormone substitution on lymphocyte subpopulations was also analyzed.
Thirteen Chinese TS women and eight age and sex-matched healthy volunteers were studied. The percentage and mean fluorescence intensity (MFI) of lymphocyte subpopulations including CD3+CD4+, CD3+CD8+, CD19-CD138+, CD4+CD25+FoxP3+ and CD4+CD8-IL17A+ cells were determined by flow cytometry. The mRNA expression of some transcription factors were detected by RT-PCR.
Compared to control, the percentage of CD3+CD4+ cells was significantly reduced (p < 0.05), while the percentage of CD19-CD138+, CD4+CD25+FoxP3+ and CD4+CD8-IL17A+ cells was significantly increased in TS patients. No difference was observed in the percentage of CD3+CD8+, CD19+ B cells between TS patients and healthy volunteers, with the similar changes in the mean fluorescence intensity of these cells. The mRNA expression of some transcription factors slightly enhanced in TS patients. Estrogen therapy did not affect the percentage of lymphocyte subpopulations.
These findings suggested that Turner syndrome might be associated with changes of lymphocyte subpopulations.
特纳综合征（TS）与细胞和体液免疫缺陷有关。然而，中国 TS 女性淋巴细胞亚群的特征尚未报道。在这项研究中，测定了 TS 患者淋巴细胞亚群的百分比和某些转录因子的 mRNA 表达。还分析了激素替代对淋巴细胞亚群的影响。
研究了 13 名中国 TS 女性和 8 名年龄和性别匹配的健康志愿者。通过流式细胞术测定包括 CD3+CD4+、CD3+CD8+、CD19-CD138+、CD4+CD25+FoxP3+和 CD4+CD8-IL17A+细胞在内的淋巴细胞亚群的百分比和平均荧光强度（MFI）。通过 RT-PCR 检测某些转录因子的 mRNA 表达。
与对照组相比，TS 患者的 CD3+CD4+细胞百分比显著降低（p<0.05），而 CD19-CD138+、CD4+CD25+FoxP3+和 CD4+CD8-IL17A+细胞的百分比显著增加。TS 患者和健康志愿者之间 CD3+CD8+、CD19+B 细胞的百分比无差异，这些细胞的平均荧光强度也有类似变化。TS 患者某些转录因子的 mRNA 表达略有增强。雌激素治疗并不影响淋巴细胞亚群的百分比。
这些发现表明特纳综合征可能与淋巴细胞亚群的变化有关。
