Rajjou Loïc, Belghazi Maya, Catusse Julie, Ogé Laurent, Arc Erwann, Godin Béatrice, Chibani Kamel, Ali-Rachidi Sonia, Collet Boris, Grappin Philippe, Jullien Marc, Gallardo Karine, Job Claudette, Job Dominique
AgroParisTech, Unité de Formation et de Recherche de Physiologie Végétale, Paris, France.
Methods Mol Biol. 2011;773:215-36. doi: 10.1007/978-1-61779-231-1_14.
The seed is the dispersal unit of plants and must survive the vagaries of the environment. It is the object of intense genetic and genomic studies because processes related to seed quality affect crop yield and the seed itself provides food for humans and animals. Presently, the general aim of postgenomics analyses is to understand the complex biochemical and molecular processes underlying seed quality, longevity, dormancy, and vigor. Due to advances in functional genomics, the recent past years have seen a tremendous progress in our understanding of several aspects of seed development and germination. Here, we describe the proteomics protocols (from protein extraction to mass spectrometry) that can be used to investigate several aspects of seed physiology, including germination and its hormonal regulation, dormancy release, and seed longevity. These techniques can be applied to the study of both model plants (such as Arabidopsis) and crops.
种子是植物的传播单位，必须经受住环境的变幻莫测。它是深入的遗传和基因组研究的对象，因为与种子质量相关的过程会影响作物产量，而且种子本身为人类和动物提供食物。目前，后基因组学分析的总体目标是了解种子质量、寿命、休眠和活力背后复杂的生化和分子过程。由于功能基因组学的进展，在过去几年里，我们对种子发育和萌发的几个方面的理解取得了巨大进展。在这里，我们描述了蛋白质组学方案（从蛋白质提取到质谱分析），这些方案可用于研究种子生理学的几个方面，包括萌发及其激素调节、休眠解除和种子寿命。这些技术可应用于模式植物（如拟南芥）和作物的研究。
