INRA, Jean-Pierre Bourgin Institute (IJPB, UMR1318 INRA-AgroParisTech), Laboratory of Excellence "Saclay Plant Sciences" (LabEx SPS); RD10, F-78026 Versailles France.
Front Plant Sci. 2012 Jun 11;3:98. doi: 10.3389/fpls.2012.00098. eCollection 2012.
The Seed Proteome Web Portal (SPWP; http://www.seed-proteome.com/) gives access to information both on quantitative seed proteomic data and on seed-related protocols. Firstly, the SPWP provides access to the 475 different Arabidopsis seed proteins annotated from two dimensional electrophoresis (2DE) maps. Quantitative data are available for each protein according to their accumulation profile during the germination process. These proteins can be retrieved either in list format or directly on scanned 2DE maps. These proteomic data reveal that 40% of seed proteins maintain a stable abundance over germination, up to radicle protrusion. During sensu stricto germination (24 h upon imbibition) about 50% of the proteins display quantitative variations, exhibiting an increased abundance (35%) or a decreasing abundance (15%). Moreover, during radicle protrusion (24-48 h upon imbibition), 41% proteins display quantitative variations with an increased (23%) or a decreasing abundance (18%). In addition, an analysis of the seed proteome revealed the importance of protein post-translational modifications as demonstrated by the poor correlation (r(2) = 0.29) between the theoretical (predicted from Arabidopsis genome) and the observed protein isoelectric points. Secondly, the SPWP is a relevant technical resource for protocols specifically dedicated to Arabidopsis seed proteome studies. Concerning 2D electrophoresis, the user can find efficient procedures for sample preparation, electrophoresis coupled with gel analysis, and protein identification by mass spectrometry, which we have routinely used during the last 12 years. Particular applications such as the detection of oxidized proteins or de novo synthesized proteins radiolabeled by [(35)S]-methionine are also given in great details. Future developments of this portal will include proteomic data from studies such as dormancy release and protein turnover through de novo protein synthesis analyses during germination.
种子蛋白质组学网络门户（SPWP；http://www.seed-proteome.com/）提供了有关定量种子蛋白质组学数据和种子相关方案的信息。首先，SPWP 提供了从二维电泳（2DE）图谱注释的 475 种不同拟南芥种子蛋白的访问。根据其在萌发过程中的积累情况，每种蛋白质都有定量数据。这些蛋白质可以以列表格式或直接在扫描的 2DE 图谱上检索。这些蛋白质组学数据表明，在萌发过程中（吸水后 24 小时），40%的种子蛋白保持稳定的丰度，直到胚根伸出。在严格意义上的萌发过程中（吸水后 24 小时），约 50%的蛋白质显示出定量变化，表现为丰度增加（35%）或减少（15%）。此外，在胚根伸出过程中（吸水后 24-48 小时），41%的蛋白质显示出定量变化，丰度增加（23%）或减少（18%）。此外，对种子蛋白质组的分析表明，蛋白质翻译后修饰的重要性，这一点可以从理论（根据拟南芥基因组预测）和观察到的蛋白质等电点之间的相关性（r(2) = 0.29）中得到证明。其次，SPWP 是专门用于拟南芥种子蛋白质组研究的协议的相关技术资源。关于 2D 电泳，用户可以找到用于样品制备、电泳与凝胶分析结合以及通过质谱法进行蛋白质鉴定的有效程序，这些程序是我们在过去 12 年中经常使用的。还详细介绍了特殊应用，如氧化蛋白或从头合成的蛋白质的检测，这些蛋白质通过[(35)S]-蛋氨酸放射性标记。该门户的未来发展将包括从休眠释放和通过萌发过程中的从头蛋白质合成分析进行蛋白质周转等研究中获得的蛋白质组学数据。
