Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Academy of Sciences, Jinan, P. R. China.
J Sep Sci. 2011 Oct;34(19):2545-58. doi: 10.1002/jssc.201000857. Epub 2011 Aug 29.
Alkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques. High-speed counter-current chromatography (HSCCC) is a liquid-liquid partition chromatography with a support-free liquid stationary phase, and is gaining more and more popularity as a viable separation technique for bioactive compounds from natural resources. In the present review, focus is placed on the separation of alkaloids by both conventional HSCCC and pH-zone-refining counter-current chromatography (CCC) techniques from herbs. The review presents the separation of over 120 different alkaloid compounds from more than 30 plant species by the conventional HSCCC and pH-zone-refining CCC. Based on the data from the literature, the proper solvent systems for the separation of alkaloids by the conventional HSCCC and pH-zone-refining CCC are also summarized.
生物碱是生物活性天然产物中分布最广的一类。由于其碱性和结构多样性，使用常规色谱技术从草药中分离和纯化生物碱常常会遇到许多实际困难。高速逆流色谱（HSCCC）是一种无支撑液的液-液分配色谱，作为一种从天然资源中分离生物活性化合物的可行分离技术，越来越受到人们的欢迎。在本综述中，重点介绍了常规 HSCCC 和 pH 区带精制逆流色谱（CCC）技术从草药中分离生物碱的方法。该综述通过常规 HSCCC 和 pH 区带精制 CCC 从 30 多种植物中分离了超过 120 种不同的生物碱化合物。根据文献中的数据，还总结了常规 HSCCC 和 pH 区带精制 CCC 分离生物碱的合适溶剂体系。
