Rudenko N V, Abbasova S G, Grishin E V
Bioorg Khim. 2011 May-Jun;37(3):354-60. doi: 10.1134/s1068162011030162.
Anthrax is the widespread acute infection disease, affecting animals and humans, refers to the bioterrorist threat agents of category A, because of the high resistance of Bacillus anthracis spores to adverse environmental factors and the ease of receiving them. We obtain a representative panel of 20 monoclonal antibodies against the key component of pathogenic exotoxins, anthrax protective antigen. Quantitative sandwich-ELISA for protective antigen with antibody obtained was developed. Six pairs of monoclonal antibodies showed the detection limit up to 1 ng/ml concentration of the protective antigen in blood serum.
炭疽是一种广泛传播的急性感染病，可感染动物和人类，由于炭疽芽孢杆菌孢子对不利环境因素具有高度抗性且易于获取，它被列为A类生物恐怖主义威胁因子。我们获得了一组具有代表性的20种单克隆抗体，这些抗体针对致病性外毒素的关键成分——炭疽保护性抗原。利用获得的抗体开发了用于检测保护性抗原的定量夹心酶联免疫吸附测定法。六对单克隆抗体对血清中保护性抗原的检测限可达1纳克/毫升浓度。
