Sastry K S R, Tuteja U, Santhosh P K, Lalitha M K, Batra H V
Division of Microbiology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, Madhya Pradesh, India 2Department of Microbiology, Christian Medical College and Hospital, Vellore, Tamil Nadu, India.
J Med Microbiol. 2003 Jan;52(Pt 1):47-49. doi: 10.1099/jmm.0.05027-0.
A simple protective antigen (PA)-reactive mAb dot-ELISA was standardized for confirmation of toxin-producing strains of Bacillus anthracis. Twenty-seven clinical isolates were collected from patients clinically suspected of having anthrax. PA was elaborated from these isolates using Casamino acids medium and the culture medium was boiled to kill the cells. PA in boiled culture supernatants was detected using a dot-ELISA. Of the 27 clinical isolates tested, PA was detected in 24 isolates. This was further confirmed by amplifying the PA gene by PCR. This testing procedure is simple to perform, specific and safer than existing procedures, which are added advantages over existing methods of identification of B. anthracis. This test system could be a valuable tool in confirming clinical and environmental isolates of B. anthracis.
一种简单的针对保护性抗原（PA）的反应性单克隆抗体斑点酶联免疫吸附测定法（dot-ELISA）被标准化，用于确认炭疽芽孢杆菌的产毒素菌株。从临床疑似患有炭疽的患者中收集了27株临床分离株。使用酪蛋白氨基酸培养基从这些分离株中制备PA，并将培养基煮沸以杀死细胞。使用斑点酶联免疫吸附测定法检测煮沸培养上清液中的PA。在测试的27株临床分离株中，在24株分离株中检测到了PA。通过聚合酶链反应（PCR）扩增PA基因进一步证实了这一点。该检测程序操作简单、特异性强且比现有程序更安全，这是相对于现有炭疽芽孢杆菌鉴定方法的额外优势。该检测系统可能是确认炭疽芽孢杆菌临床和环境分离株的有价值工具。
