Amin Iram, Idrees Muhammad, Awan Zunaira, Shahid Muhammad, Afzal Samia, Hussain Abrar
Division of Molecular Virology & Molecular Diagnostics, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore-53500, Pakistan.
BMC Res Notes. 2011 Sep 8;4:332. doi: 10.1186/1756-0500-4-332.
Nucleic acid amplification assays including PCR have revolutionized the detection of Mycobacterium tuberculosis (MTB). Tuberculosis spread to almost every organ of the body and is characterized on the basis of localization of infection. Therefore, different types of body fluids and tissues can be used for the detection of MTB.From 2004 to 2010 total 766 different types of smear negative samples from patients, clinically suspected for tuberculosis were received and investigated at Division of Molecular Diagnostics, University of the Punjab Lahore for the diagnosis of tuberculosis. Mycobacterial DNA was extracted followed by PCR amplification.
A total of 356 (46.5%) samples were found positive by PCR for MTB. These included; serum (4.8%), blood (36.3%), urine (46.6%), cerebro spinal fluid (CSF) (42.1%), ascetic fluid (67.6%), pleural fluid (52%), pericardial fluid (30%), pus (38.6%), bone marrow (60%), sputum (38.8%) and bronchoalveolar lavage (BAL) (70%). Over all there was no significant difference in males and females neither in different age groups for the identification of MTB.
We conclude that PCR is a useful and sensitive tool for the early diagnosis of MTB in variety of clinical samples.
包括聚合酶链反应（PCR）在内的核酸扩增检测方法彻底改变了结核分枝杆菌（MTB）的检测方式。结核病可传播至身体的几乎每个器官，并根据感染部位进行分类。因此，不同类型的体液和组织可用于MTB的检测。2004年至2010年期间，旁遮普大学拉合尔分校分子诊断科共接收并研究了766份临床疑似结核病患者的不同类型涂片阴性样本，以诊断结核病。提取分枝杆菌DNA，随后进行PCR扩增。
共有356份（46.5%）样本经PCR检测MTB呈阳性。这些样本包括：血清（4.8%）、血液（36.3%）、尿液（46.6%）、脑脊液（CSF）（42.1%）、腹水（67.6%）、胸水（52%）、心包积液（30%）、脓液（38.6%）、骨髓（60%）、痰液（38.8%）和支气管肺泡灌洗（BAL）液（70%）。总体而言，在MTB的鉴定中，男性和女性以及不同年龄组之间均无显著差异。
我们得出结论，PCR是一种用于多种临床样本中MTB早期诊断的有用且敏感的工具。
