重组人骨形态发生蛋白-2对成骨的促进作用
Accelerators of osteogenesis by recombinant human bone morphogenetic protein-2.
作者信息
Okubo Yasunori, Kusumoto Kenji, Bessho Kazuhisa
机构信息
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
出版信息
Drug Target Insights. 2007;2:55-60. Epub 2007 Feb 21.
Bone morphogenetic protein (BMP) appears to be one of the most promising cytokine and for clinical use in reconstructive surgery for bony defects and augmentation. To evaluate the effect of basic fibroblast growth factor (bFGF), FK506, elcatonin, and hyperbaric oxygenation (HBO) on osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2), 2 or 5 μg of rhBMP-2 was implanted into intramuscular sites of rats. At 21 days after implantation, the osteoinductive activity in the treatment group and control group was compared radiographically, biochemically, and histologically. The amount of new bone in the treatment group was significantly greater than that in the control group. The alkaline phosphatase activity and calcium content in the treatment group were significantly higher than those in the control group. These results suggest that bFGF, FK506, elcatonin, and HBO accelerated the activity and rate of osteoinduction by rhBMP2. These results may be useful when BMP is applied clinically in near future.
骨形态发生蛋白（BMP）似乎是最有前景的细胞因子之一，可用于骨缺损修复和骨增量的重建手术临床应用。为了评估碱性成纤维细胞生长因子（bFGF）、FK506、降钙素和高压氧（HBO）对重组人骨形态发生蛋白-2（rhBMP-2）骨诱导作用的影响，将2或5μg的rhBMP-2植入大鼠肌肉内。植入后21天，通过影像学、生化和组织学方法比较治疗组和对照组的骨诱导活性。治疗组的新骨量明显多于对照组。治疗组的碱性磷酸酶活性和钙含量明显高于对照组。这些结果表明，bFGF、FK506、降钙素和HBO加速了rhBMP2的骨诱导活性和速率。这些结果在不久的将来BMP临床应用时可能会有用。
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