Berg Daniela, Malinowsky Katharina, Reischauer Bilge, Wolff Claudia, Becker Karl-Friedrich
Institut für Pathologie, Technische Universität München, Munich, Germany.
Methods Mol Biol. 2011;785:109-22. doi: 10.1007/978-1-61779-286-1_8.
Formalin-fixed and paraffin-embedded (FFPE) tissues are used routinely everyday in hospitals world-wide for histopathological diagnosis of diseases like cancer. Due to formalin-induced cross-linking of proteins, FFPE tissues present a particular challenge for proteomic analysis. Nevertheless, there has been recent progress for extraction-based protein analysis in these tissues. Novel tools developed in the last few years are urgently needed because precise protein biomarker quantification in clinical FFPE tissues will be crucial for treatment decisions and to assess success or failure of current and future personalized molecular therapies. Furthermore, they will help to conceive why only a subset of patients responds to individualized treatments. Reverse phase protein array (RPPA) is a very promising new technology for quick and simultaneous analysis of many patient samples allowing relative and absolute protein quantifications. In this chapter, we show how protein extraction from FFPE tissues might facilitate the implementation of RPPA for therapy decisions and discuss challenges for application of RPPA in clinical trials and routine settings.
在世界各地的医院里，福尔马林固定石蜡包埋（FFPE）组织每天都被常规用于癌症等疾病的组织病理学诊断。由于福尔马林会诱导蛋白质发生交联，FFPE组织在蛋白质组学分析中面临着特殊的挑战。然而，近年来在这些组织基于提取的蛋白质分析方面取得了进展。由于在临床FFPE组织中精确的蛋白质生物标志物定量对于治疗决策以及评估当前和未来个性化分子疗法的成败至关重要，因此迫切需要在过去几年中开发的新工具。此外，它们将有助于理解为什么只有一部分患者对个体化治疗有反应。反相蛋白质阵列（RPPA）是一种非常有前景的新技术，可用于快速同时分析许多患者样本，实现蛋白质的相对和绝对定量。在本章中，我们展示了从FFPE组织中提取蛋白质如何促进RPPA用于治疗决策的实施，并讨论了RPPA在临床试验和常规环境中应用所面临的挑战。
