McWilliam Iain, Chong Kwan Marisa, Hall Duncan
Arrayjet Ltd., MIC, Roslin, UK.
Methods Mol Biol. 2011;785:345-61. doi: 10.1007/978-1-61779-286-1_23.
A significant proportion of protein microarray researchers would like the arrays they develop to become widely used research, screening, validation or diagnostic devices. For this to be achievable the arrays must be compatible with high-throughput techniques that allow manufacturing scale production. In order to simplify the transition from laboratory bench to market, Arrayjet have developed a range of inkjet microarray printers, which, at one end of the scale, are suitable for R&D and, at the other end, are capable of true high-throughput array output. To maintain scalability, all Arrayjet microarray printers utilise identical core technology comprising a JetSpyder™ liquid handling adaptor, which enables automated loading of an industry standard inkjet printhead compatible with non-contact on-the-fly printing. This chapter contains a detailed explanation of Arrayjet technology followed by a historical look at the development of inkjet technologies for protein microarray production. The method described subsequently is a simple example of an antibody array printed onto nitrocellulose-coated slides with specific detection with fluorescently labelled IgG. The method is linked to a notes section with advice on best practice and sources of useful information for protein microarray production using inkjet technology.
相当一部分蛋白质微阵列研究人员希望他们开发的阵列能够成为广泛应用于研究、筛选、验证或诊断的设备。要实现这一点，阵列必须与允许进行大规模生产的高通量技术兼容。为了简化从实验室工作台到市场的过渡，Arrayjet公司开发了一系列喷墨微阵列打印机，这些打印机一方面适用于研发，另一方面能够实现真正的高通量阵列输出。为了保持可扩展性，所有Arrayjet微阵列打印机都采用相同的核心技术，包括一个JetSpyder™液体处理适配器，该适配器能够自动加载与非接触式实时打印兼容的行业标准喷墨打印头。本章详细解释了Arrayjet技术，随后回顾了用于蛋白质微阵列生产的喷墨技术的发展历程。随后描述的方法是一个简单的例子，即把抗体阵列打印到硝酸纤维素包被的载玻片上，并用荧光标记的IgG进行特异性检测。该方法与一个注释部分相关联，其中提供了关于使用喷墨技术进行蛋白质微阵列生产的最佳实践和有用信息来源的建议。
