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B 群脑膜炎奈瑟菌荚膜蛋白抗原的免疫原性分析。

Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis.

机构信息

Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, M13 9PL, UK.

National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK.

出版信息

Sci Rep. 2019 May 2;9(1):6843. doi: 10.1038/s41598-019-43139-0.

DOI:10.1038/s41598-019-43139-0
PMID:31048732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6497663/
Abstract

Outer membrane vesicle (OMV)- based vaccines have been used to provide strain-specific protection against capsular group B Neisseria meningitidis infections, but the full breadth of the immune response against the components of the OMV has not been established. Sera from adults vaccinated with an OMV vaccine were used to screen 91 outer membrane proteins (OMPs) incorporated in an antigen microarray panel. Antigen-specific IgG levels were quantified pre-vaccination, and after 12 and 18 weeks. These results were compared with IgG levels from mice vaccinated with the same OMV vaccine. The repertoires of highly responding antigens in humans and mice overlapped, but were not identical. The highest responding antigens to human IgG comprised four integral OMPs (PorA, PorB, OpcA and PilQ), a protein which promotes the stability of PorA and PorB (RmpM) and two lipoproteins (BamC and GNA1162). These observations will assist in evaluating the role of minor antigen components within OMVs in providing protection against meningococcal infection. In addition, the relative dominance of responses to integral OMPs in humans emphasizes the importance of this subclass and points to the value of maintaining conformational epitopes from integral membrane proteins in vaccine formulations.

摘要

基于外膜囊泡（OMV）的疫苗已被用于提供针对荚膜组 B 脑膜炎奈瑟菌感染的菌株特异性保护，但尚未确定针对 OMV 成分的免疫反应的全貌。用 OMV 疫苗接种的成年人的血清用于筛选包含在抗原微阵列面板中的 91 种外膜蛋白（OMP）。在接种疫苗前、接种后 12 周和 18 周时定量测定抗原特异性 IgG 水平。将这些结果与用相同 OMV 疫苗接种的小鼠的 IgG 水平进行比较。人类和小鼠中高反应性抗原的反应谱重叠，但不完全相同。对人 IgG 反应最高的抗原包括四个完整的 OMP（PorA、PorB、OpcA 和 PilQ）、一种促进 PorA 和 PorB 稳定性的蛋白（RmpM）以及两种脂蛋白（BamC 和 GNA1162）。这些观察结果将有助于评估 OMV 中次要抗原成分在提供针对脑膜炎球菌感染的保护方面的作用。此外，人类对完整 OMP 的反应相对优势强调了这一类的重要性，并指出在疫苗配方中保持完整膜蛋白的构象表位的价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/159750048146/41598_2019_43139_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/bd34ad0d35ed/41598_2019_43139_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/74a99bedadeb/41598_2019_43139_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/b1b9d86c3ba2/41598_2019_43139_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/af66b8a31581/41598_2019_43139_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/159750048146/41598_2019_43139_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/bd34ad0d35ed/41598_2019_43139_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/74a99bedadeb/41598_2019_43139_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/b1b9d86c3ba2/41598_2019_43139_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/af66b8a31581/41598_2019_43139_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4be/6497663/159750048146/41598_2019_43139_Fig5_HTML.jpg

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