National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892-4330, USA.
Adv Exp Med Biol. 2011;720:27-38. doi: 10.1007/978-1-4614-0254-1_3.
The human oral squamous epithelium plays an important role in maintaining a barrier function against mechanical, physical, and pathological injury. However, the self-renewing cells residing on the basement membrane of the epithelium can give rise to oral squamous cell carcinomas (OSCC), now the sixth most common cancer in the developed world, which is still associated with poor prognosis. This is due, in part, to the limited availability of well-defined culture systems for studying oral epithelial cell biology, which could advance our understanding of the molecular basis of OSCC. Here, we describe methods to successfully isolate large cultures of human oral epithelial cells and fibroblasts from small pieces of donor tissues for use in techniques such as three-dimensional cultures and animal grafts to validate genes suspected of playing a role in OSCC development and progression. Finally, the use of isolated oral epithelial cells in generating iPS cells is discussed which holds promise in the field of oral regenerative medicine.
人类口腔鳞状上皮在维持对机械、物理和病理性损伤的屏障功能方面发挥着重要作用。然而，位于上皮基底膜上的自我更新细胞可以引发口腔鳞状细胞癌（OSCC），如今这种癌症在发达国家已排名第六，且预后仍然较差。部分原因是，目前用于研究口腔上皮细胞生物学的明确培养体系有限，这可能会增进我们对 OSCC 分子基础的理解。在这里，我们描述了从小块供体组织中成功分离大量人口腔上皮细胞和成纤维细胞的方法，可用于三维培养和动物移植等技术，以验证疑似在 OSCC 发生和发展中起作用的基因。最后，还讨论了分离的口腔上皮细胞在生成 iPS 细胞中的应用，这在口腔再生医学领域具有广阔的应用前景。
