Asanuma Hiroyuki, Nishioka Hidenori, Ishikawa Teruchika, Liang Xingguo
Nagoya University, Nagoya, Japan.
Curr Protoc Nucleic Acid Chem. 2011 Sep;Chapter 4:Unit 4.45.1-18. doi: 10.1002/0471142700.nc0445s46.
This unit describes synthetic procedures of photoresponsive DNA via a phosphoramidite monomer composed of D-threoninol as a scaffold and 4-carboxy-2',6'-dimethylazobenzene or 4-carboxy-2'-methylazobenzene that works as a photoswitch more efficiently than previous nonmodified azobenzene (4-phenylazobenzoic acid). With these newly modified-azobenzenes, photoregulatory efficiency of DNA hybridization can be greatly improved. Furthermore, thermal stability of cis-azobenzene of 4-carboxy-2',6'-dimethylazobenzene remarkably increases compared with the previous non-modified azobenzene.
本单元描述了通过由D-苏糖醇作为支架和4-羧基-2',6'-二甲基偶氮苯或4-羧基-2'-甲基偶氮苯组成的亚磷酰胺单体合成光响应性DNA的过程，这些偶氮苯作为光开关比以前未修饰的偶氮苯（4-苯基偶氮苯甲酸）更有效。使用这些新修饰的偶氮苯，可以大大提高DNA杂交的光调节效率。此外，与以前未修饰的偶氮苯相比，4-羧基-2',6'-二甲基偶氮苯的顺式偶氮苯的热稳定性显著提高。
