Department of Environmental Engineering and Science, Feng Chia University, Taichung, Chinese Taiwan.
Water Sci Technol. 2011;63(9):1849-54. doi: 10.2166/wst.2011.390.
Anaerobic digestion is an effective technology to convert cellulosic wastes to methane and hydrogen. Heat-treatment is a well known method to inhibit hydrogen-consuming bacteria in using anaerobic mixed cultures for seeding. This study aims to investigate the effects of heat-treatment temperature and time on activated sludge for fermentative hydrogen production from alpha-cellulose by response surface methodology. Hydrogen and methane production was evaluated based on the production rate and yield (the ability of converting cellulose into hydrogen and methane) with heat-treated sludge as the seed at various temperatures (60-97 degrees C) and times (20-60 min). Batch experiments were conducted at 55 degrees C and initial pH of 8.0. The results indicate that hydrogen and methane production yields peaked at 4.3 mmol H2/g cellulose and 11.6 mmol CH4/g cellulose using the seed activated sludge that was thermally treated at 60 degrees C for 40 min. These parameter values are higher than those of no-treatment seed (HY 3.6 mmol H2/g cellulose and MY 10.4 mmol CH4/g cellulose). The maximum hydrogen production rate of 26.0 mmol H2/L/d and methane production rate of 23.2 mmol CH4/L/d were obtained for the seed activated sludge that was thermally treated at 70 degrees C for 50 min and 60 degrees C for 40 min, respectively.
厌氧消化是一种将纤维素废物转化为甲烷和氢气的有效技术。热处理是一种众所周知的方法，用于抑制使用厌氧混合培养物作为种子时消耗氢气的细菌。本研究旨在通过响应面法研究热处理温度和时间对用于从α-纤维素发酵生产氢气的活性污泥的影响。根据产氢率和产甲烷率（将纤维素转化为氢气和甲烷的能力）来评估氢气和甲烷的产生，其中以不同温度（60-97°C）和时间（20-60 分钟）处理的污泥作为种子。批处理实验在 55°C 和初始 pH 值为 8.0 的条件下进行。结果表明，使用在 60°C 下热处理 40 分钟的种子活性污泥，氢气和甲烷的产率分别达到 4.3mmol H2/g 纤维素和 11.6mmol CH4/g 纤维素的峰值。这些参数值高于未经处理的种子（HY 3.6mmol H2/g 纤维素和 MY 10.4mmol CH4/g 纤维素）。对于在 70°C 下热处理 50 分钟和 60°C 下热处理 40 分钟的种子活性污泥，分别获得了 26.0mmol H2/L/d 的最大产氢率和 23.2mmol CH4/L/d 的最大产甲烷率。
