Fahmi A I, Nagaty H H, Eissa R A, Hassan M M
Department of Biotechnology, Faculty of Sciences, Taif University, Kingdom of Saudi Arabia.
Pak J Biol Sci. 2011 Mar 15;14(6):385-91. doi: 10.3923/pjbs.2011.385.391.
The adverse effects of sea water salinity on number of nodules, nitrogen content, nitroginase activity, Chlorophyll a and b content, proline accumulation and protein pattern of faba bean plants (Vicia faba commercial cultivar Nubaria 1) were investigated. Faba bean plants were irrigated with sea water at 20, 25, 30, 40 and 50% concentrations and inoculated with rhizobial isolate ARC307 or with gamma rays treated isolates namely; ARC1, ARC2, ARC3, ARC4, ARC5, ARC6 and ARC7. Nodules number, nitrogen content, nitroginase activity and chlorophyll a and b content parameters were decreased by increasing sea water salinity with all used isolates, while proline accumulation parameter increased. At the same time, ARC2 isolate showed the highest values for these parameters above all isolates including the parental isolate ARC307 at all studied concentrations except for proline accumulation parameter, it was the least. Therefore, ARC2 considered as a promising isolate for salt tolerance. Salinity enhanced the occurrence of particular novel proteins in faba bean plants infected with ARC2 isolate.
研究了海水盐度对蚕豆植株(商业品种努巴里亚1号蚕豆)根瘤数量、氮含量、固氮酶活性、叶绿素a和b含量、脯氨酸积累及蛋白质图谱的不利影响。用浓度为20%、25%、30%、40%和50%的海水灌溉蚕豆植株,并接种根瘤菌分离株ARC307或经γ射线处理的分离株,即ARC1、ARC2、ARC3、ARC4、ARC5、ARC6和ARC7。随着海水盐度的增加,所有使用的分离株处理下的根瘤数量、氮含量、固氮酶活性以及叶绿素a和b含量参数均下降,而脯氨酸积累参数增加。同时,除脯氨酸积累参数外,在所有研究浓度下,ARC2分离株在这些参数上的值均高于所有分离株,包括亲本分离株ARC307,脯氨酸积累参数是最低的。因此,ARC2被认为是一种有前景的耐盐分离株。盐度增加了感染ARC2分离株的蚕豆植株中特定新蛋白质的出现。