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本文引用的文献

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The influenza virus haemagglutinnation inhibition by antibody to host material.宿主物质抗体对流感病毒血凝反应的抑制作用
Acta Pathol Microbiol Scand. 1963;57:317-30. doi: 10.1111/j.1699-0463.1963.tb05101.x.
2
Characterization of an established line of canine kidney cells (MDCK).已建立的犬肾细胞系（MDCK）的特性描述。
Proc Soc Exp Biol Med. 1966 Jul;122(3):931-5. doi: 10.3181/00379727-122-31293.
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The structure of influenza viruses. IV. Chemical studies of the host antigen.流感病毒的结构。IV. 宿主抗原的化学研究。
Virology. 1966 Sep;30(1):104-15. doi: 10.1016/s0042-6822(66)81014-0.
4
Replication and plaque assay of influenza virus in an established line of canine kidney cells.流感病毒在已建立的犬肾细胞系中的复制及蚀斑测定
Appl Microbiol. 1968 Apr;16(4):588-94. doi: 10.1128/am.16.4.588-594.1968.
5
Search for tumor-specific immune reactions in Burkitt lymphoma patients by the membrane immunofluorescence reaction.通过膜免疫荧光反应在伯基特淋巴瘤患者中寻找肿瘤特异性免疫反应。
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1628-35. doi: 10.1073/pnas.55.6.1628.
6
Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin.在胰蛋白酶存在的情况下，对甲型流感病毒进行蚀斑测定及在已建立的犬肾细胞系（MDCK）中进行初次分离。
Med Microbiol Immunol. 1975 Dec 30;162(1):9-14. doi: 10.1007/BF02123572.
7
Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses.用于乙型流感病毒分离和蚀斑测定的永生化犬肾（MDCK）细胞。
Med Microbiol Immunol. 1975 Dec 30;162(1):23-7. doi: 10.1007/BF02123574.

用于分离呼吸道病毒的犬肾细胞系。

Canine kidney cell line for isolation of respiratory viruses.

作者信息

Meguro H, Bryant J D, Torrence A E, Wright P F

出版信息

J Clin Microbiol. 1979 Feb;9(2):175-9. doi: 10.1128/jcm.9.2.175-179.1979.

DOI:10.1128/jcm.9.2.175-179.1979
PMID:219021
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272985/
Abstract

By means of a continuous canine kidney cell line (MDCK), influenza viruses were rapidly isolated from specimens collected from patients with respiratory disease. The cell line proved more sensitive than either eggs or rhesus monkey cells for currently circulating influenza A and B strains. Influenza viruses caused a distinct cytopathology within 5 days of inoculation if trypsin-ethylenediamine-tetraacetic acid was incorporated into the medium. Sufficient hemagglutinin was produced on the initial tissue culture passage to allow direct identification of isolates by hemagglutinin inhibition tests. A variety of other respiratory viruses replicated in MDCK, and over a 10-month period 211 of 600 specimens (35%) yielded viruses.

摘要

利用连续传代的犬肾细胞系（MDCK），从呼吸道疾病患者采集的标本中快速分离出流感病毒。对于目前流行的甲型和乙型流感毒株，该细胞系比鸡胚或恒河猴细胞更敏感。如果在培养基中加入胰蛋白酶 - 乙二胺四乙酸，流感病毒在接种后5天内会引起明显的细胞病变。在初次组织培养传代时就能产生足够的血凝素，以便通过血凝抑制试验直接鉴定分离株。多种其他呼吸道病毒也能在MDCK中复制，在10个月的时间里，600份标本中有211份（35%）检出病毒。