Meguro H, Bryant J D, Torrence A E, Wright P F
J Clin Microbiol. 1979 Feb;9(2):175-9. doi: 10.1128/jcm.9.2.175-179.1979.
By means of a continuous canine kidney cell line (MDCK), influenza viruses were rapidly isolated from specimens collected from patients with respiratory disease. The cell line proved more sensitive than either eggs or rhesus monkey cells for currently circulating influenza A and B strains. Influenza viruses caused a distinct cytopathology within 5 days of inoculation if trypsin-ethylenediamine-tetraacetic acid was incorporated into the medium. Sufficient hemagglutinin was produced on the initial tissue culture passage to allow direct identification of isolates by hemagglutinin inhibition tests. A variety of other respiratory viruses replicated in MDCK, and over a 10-month period 211 of 600 specimens (35%) yielded viruses.
利用连续传代的犬肾细胞系(MDCK),从呼吸道疾病患者采集的标本中快速分离出流感病毒。对于目前流行的甲型和乙型流感毒株,该细胞系比鸡胚或恒河猴细胞更敏感。如果在培养基中加入胰蛋白酶 - 乙二胺四乙酸,流感病毒在接种后5天内会引起明显的细胞病变。在初次组织培养传代时就能产生足够的血凝素,以便通过血凝抑制试验直接鉴定分离株。多种其他呼吸道病毒也能在MDCK中复制,在10个月的时间里,600份标本中有211份(35%)检出病毒。