Sun Wei, Li Zi-rong, Yang Yu-Run, Shi Zhen-cai, Wang Bailiang, Liu Bingli, Shi Shaohui
Center for Osteonecrosis and Joint Preserving and Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing, China.
Orthopedics. 2011 Sep 9;34(9):e530-4. doi: 10.3928/01477447-20110714-07.
Synchrotron radiation light is 1 of 4 artificial light sources, the others being electric light, X-ray, and laser. Phase-contrast imaging with hard X-ray has achieved wide application in many scientific fields, such as biomedicine and material science. This article compares the effectiveness of nanohydroxyapatite/collagen (nHAC) and autologous mesenchymal stem cell for the repair of defects in a rabbit model with osteonecrosis of the femoral head under the monitoring of phase-contrast imaging with synchrotron hard X-ray. We established models of bilateral osteonecrosis of the femoral head defect using New Zealand rabbits and divided them into 3 groups. Imaging techniques such as phase-contrast imaging and diffraction enhanced imaging with synchrotron hard X-ray were applied to assess the degradation and repair process of nHAC and mesenchymal stem cell at 4, 8, and 12 weeks postoperatively. We found phase-contrast imaging with synchrotron hard X-ray displayed the reparative process of the bone defect, degradation of nHAC, and osteocyte substitution. There were significant differences in the repair of the bone defect and osteogenesis in groups B and C compared with group A (control). Osteogenesis was more significant in group C. We provided experimental data for the development and application of synchrotron hard X-ray imaging techniques and concluded that phase-contrast microimaging with synchrotron hard X-ray displays the reparative process of bone tissue at a micro-level and plays an important role in the development of tissue engineering.
同步辐射光是四种人造光源之一，其他三种是电灯、X射线和激光。硬X射线相衬成像已在生物医学和材料科学等许多科学领域得到广泛应用。本文在同步辐射硬X射线相衬成像监测下，比较纳米羟基磷灰石/胶原蛋白（nHAC）和自体间充质干细胞修复兔股骨头坏死模型缺损的效果。我们用新西兰兔建立双侧股骨头坏死缺损模型，并将其分为3组。术后4周、8周和12周，应用同步辐射硬X射线相衬成像和衍射增强成像等成像技术评估nHAC和间充质干细胞的降解和修复过程。我们发现同步辐射硬X射线相衬成像显示了骨缺损的修复过程、nHAC的降解以及骨细胞替代。与A组（对照组）相比，B组和C组在骨缺损修复和成骨方面存在显著差异。C组的成骨更显著。我们为同步辐射硬X射线成像技术的开发和应用提供了实验数据，并得出结论，同步辐射硬X射线相衬显微成像在微观水平上显示了骨组织的修复过程，在组织工程发展中发挥着重要作用。
