INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.
Tissue Eng Part A. 2012 Jan;18(1-2):137-46. doi: 10.1089/ten.TEA.2011.0294. Epub 2011 Sep 15.
Tissue engineering constitutes a promising alternative technology to transplantation medicine by creating viable substitutes for failing tissues or organs. The ability to manipulate and reconstitute tissue function has tremendous clinical implications and will most likely play a key role in cell and gene therapies in the coming years. In the present work, a novel injectable and biodegradable biomaterial is reported that could be injected on the human body with a surgical syringe. The material prepared is a blend of polycaprolactone (PCL), a biodegradable and elastic biomedical polymer, and sebacic acid, a natural polymer part of castor oil with low molecular weight to accelerate the slow degradation rate of PCL. The biocompatibility of the blend was evaluated in vitro and its in vivo behavior was also assessed through subcutaneous and bone implantation in rats to evaluate its tissue-forming ability and degradation rate. The results allowed the conclusion that the gel is biocompatible, promotes the differentiation of mesenchymal stem cells, and presents an adequate degradation rate for use in bone tissue engineering. In vivo the gel blends promoted tissue regeneration and adverse reactions were not observed on subcutaneous and bone implants.
组织工程学通过为衰竭的组织或器官创造可行的替代品，构成了移植医学的一种有前途的替代技术。操纵和重建组织功能的能力具有巨大的临床意义，并且很可能在未来几年的细胞和基因治疗中发挥关键作用。在本工作中，报道了一种新型的可注射和可生物降解的生物材料，该材料可以用手术注射器注射到人体上。所制备的材料是聚己内酯（PCL）和癸二酸的混合物，PCL 是一种可生物降解和弹性的生物医学聚合物，癸二酸是蓖麻油的天然低分子量聚合物部分，可加速 PCL 的缓慢降解速率。通过体外评估了混合物的生物相容性，并通过在大鼠的皮下和骨内植入来评估其体内行为，以评估其组织形成能力和降解率。结果表明，该凝胶具有生物相容性，可促进间充质干细胞的分化，并具有足够的降解速率，可用于骨组织工程。体内实验中，凝胶混合物促进了组织再生，在皮下和骨植入物中未观察到不良反应。
