Reproductive Biology Department, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Sección XVI, Tlalpan 14000 México, DF, México.
Br J Nutr. 2012 Jun;107(11):1562-5. doi: 10.1017/S0007114511004855. Epub 2011 Sep 9.
Maternal low-protein (LP) diets programme β-cell secretion, potentially altering the emergence of ageing of offspring pancreatic function. We hypothesised that isolated pancreatic islet β-cell secretory responses are blunted in offspring exposed to LP during development and age-related reduction is influenced by the developmental stage of exposure to decreased nutrition. We studied male offspring of rats fed control (C) or LP protein (R) diets in pregnancy, first letter and/or lactation second letter of CC, RR, CR or RC groups. Serum glucose, insulin and homeostatic model assessment (HOMA) were measured. Pancreatic islets were isolated and in vitro insulin secretion quantified in low (LG - 5 mM) or high glucose (HG - 11 mM). Body weight and serum values between groups were similar at all ages. Insulin and HOMA rose with age and were highest at postnatal day (PND) 450 in all groups. At PND 36, insulin secretion was greatest in RR and RC. Only CC increased insulin secretion to HG. By PND 110, restricted groups responded less to LG but increased secretion to HG. By PND 450, CC offspring alone increased secretion to HG. Despite minimal differences in circulating insulin and glucose, reduced maternal protein intake affected insulin secretion at all ages. In addition, ageing reduced function in all R groups compared with CC by PND 110 and further by PND 450 most markedly in RC. We conclude that maternal LP diet during pregnancy and/or lactation impairs offspring insulin secretory response to a glucose challenge and alters the trajectory of ageing of pancreatic insulin secretion.
母体低蛋白（LP）饮食方案会影响β细胞的分泌，从而可能改变后代胰腺功能的衰老。我们假设，在发育过程中暴露于 LP 的后代的胰岛β细胞分泌反应会减弱，而与营养减少的发育阶段相关的与年龄相关的减少会受到影响。我们研究了在妊娠、哺乳期第一字母和/或哺乳期第二字母中接受对照（C）或 LP 蛋白（R）饮食的雄性后代大鼠的胰岛。测量血清葡萄糖、胰岛素和稳态模型评估（HOMA）。分离胰岛并在低（LG-5mM）或高葡萄糖（HG-11mM）下量化体外胰岛素分泌。所有年龄段各组之间的体重和血清值相似。胰岛素和 HOMA 随年龄增长而升高，所有组在产后第 450 天最高。在 PND36 时，RR 和 RC 组的胰岛素分泌最大。只有 CC 组增加了对 HG 的胰岛素分泌。到 PND110 时，受限制的组对 LG 的反应较小，但对 HG 的分泌增加。到 PND450 时，只有 CC 组后代增加了对 HG 的分泌。尽管循环胰岛素和葡萄糖差异很小，但减少母体蛋白质摄入会影响所有年龄段的胰岛素分泌。此外，与 CC 相比，老化会降低所有 R 组在 PND110 时和在 PND450 时的胰岛素分泌功能，在 RC 中最为明显。我们得出结论，母体在妊娠和/或哺乳期 LP 饮食会损害后代对葡萄糖刺激的胰岛素分泌反应，并改变胰腺胰岛素分泌的衰老轨迹。
