Bleeg H S
Department of Oral Biology, Royal Dental College, Arhus, Denmark.
Scand J Dent Res. 1990 Jun;98(3):235-41. doi: 10.1111/j.1600-0722.1990.tb00967.x.
Proteolytic degradation of collagen normally requires specific collagenases of either mammalian or microbial origin. We have observed, among many detergents, a unique effect of sodium dodecyl (= lauryl) sulfate after preincubation of insoluble collagen (type I) with detergent. When treated fibrils were isolated and resuspended in detergent-free buffer, they were very sensitive to cleavage by non-specific proteases like trypsin, elastase, and subtilisin. We suggest that dodecyl sulfate causes a structural change in the collagen molecule which abrogates the resistance of collagen to most proteolytic enzymes. These observations may have implications for collagen and connective tissue research in general and especially to periodontal research. Lauryl sulfate (2-5%) is included in the majority of toothpaste formulations as a foaming agent, and our results again raise the question whether toothbrushing with lauryl sulfate may accelerate periodontal destruction by synergism with bacterial and host proteases. It may also bind to the collagen in exposed root surfaces and thus affect the stability of their hard tissues.
胶原蛋白的蛋白水解降解通常需要源自哺乳动物或微生物的特定胶原酶。在众多去污剂中，我们观察到，将不溶性I型胶原蛋白与去污剂预孵育后，十二烷基硫酸钠（即月桂基硫酸钠）具有独特的作用。当将经处理的原纤维分离并重悬于不含去污剂的缓冲液中时，它们对胰蛋白酶、弹性蛋白酶和枯草杆菌蛋白酶等非特异性蛋白酶的切割非常敏感。我们认为，十二烷基硫酸盐会导致胶原蛋白分子发生结构变化，从而消除胶原蛋白对大多数蛋白水解酶的抗性。这些观察结果可能对一般的胶原蛋白和结缔组织研究，尤其是对牙周研究具有启示意义。大多数牙膏配方中都含有2%-5%的月桂基硫酸盐作为发泡剂，我们的研究结果再次提出了一个问题，即使用含月桂基硫酸盐的牙膏刷牙是否会与细菌和宿主蛋白酶协同作用，加速牙周组织的破坏。它还可能与暴露牙根表面的胶原蛋白结合，从而影响其硬组织的稳定性。
