Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 143-701, Republic of Korea.
Bioorg Med Chem Lett. 2011 Oct 15;21(20):6054-7. doi: 10.1016/j.bmcl.2011.08.065. Epub 2011 Aug 22.
Schizandra chinensis has been known to have five predominant tastes: salty, sweet, sour, astringent, and bitter. It has also been shown to have various effects on the cardiovascular system, gastrointestinal system, anti-inflammatory, central nervous system, endocrine system, and stress protect. However, its anti-cancer activity on colon carcinoma HCT-116 cells has not been yet been examined. Thus, in this study, we attempted to isolate a compound from Schisandra chinensis that induced apoptosis in HCT-116 cells. An active compound was found and identified to be Gomisin A. It displayed apoptotic activity through caspase-7 cleavage in colon carcinoma HCT-116 cells. In addition, we further assessed the effects of this compound using long-term survival clonogenic assay with HCT116 cells.
咸、甜、酸、涩、苦。它还对心血管系统、胃肠道系统、抗炎、中枢神经系统、内分泌系统和应激保护具有多种作用。然而，它对结肠癌 HCT-116 细胞的抗癌活性尚未得到研究。因此，在这项研究中，我们试图从五味子中分离出一种诱导 HCT-116 细胞凋亡的化合物。发现并鉴定出一种活性化合物为五味子甲素。它通过切割半胱天冬氨酸蛋白酶-7在结肠癌 HCT-116 细胞中显示出凋亡活性。此外，我们还使用长期生存集落形成测定法进一步评估了该化合物对 HCT116 细胞的影响。
