Ko Young Hyun, Jeong Miran, Jang Dae Sik, Choi Jung-Hye
Division of Molecular Biology, College of Pharmacy, Kyung Hee University, Seoul 02447, Korea.
Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul 02447, Korea.
Life (Basel). 2021 Aug 21;11(8):858. doi: 10.3390/life11080858.
The fruits of (Schisandra berries) are used as health food supplements and popular food ingredients in East Asia. Lignans, major and characteristic polyphenol compounds of Schisandra berries, possess various biological activities, including hepatoprotective and anticancer effects. However, the biological activities of gomisin L1, a lignan isolated from Schisandra berries, are less to be investigated. In this study, the antitumor activity of gomisin L1 and its underlying molecular mechanism in human ovarian cancer cells were investigated. Gomisin L1 exhibited potent cytotoxic activity against A2780 and SKOV3 ovarian cancer cells. Flow cytometry analysis revealed that the growth inhibitory effects of gomisin L1 were mediated by the induction of apoptosis. Furthermore, gomisin L1 induced an increase in intracellular reactive oxygen species (ROS) levels, and the antioxidant -acetyl cysteine significantly negated gomisin L1-induced cell death. Moreover, inhibition of NADPH oxidase (NOX) using an inhibitor and siRNA attenuated gomisin L1-induced death of, and ROS production in, human ovarian cancer cells. Taken together, these data indicate that the lignan gomisin L1 from Schisandra berries induces apoptotic cell death by regulating intracellular ROS production via NOX.
五味子果实作为保健食品补充剂和东亚流行的食品成分被广泛使用。木脂素是五味子果实中的主要且具有特征性的多酚化合物，具有多种生物活性，包括保肝和抗癌作用。然而，从五味子果实中分离出的木脂素戈米辛L1的生物活性却鲜有研究。在本研究中，我们对戈米辛L1在人卵巢癌细胞中的抗肿瘤活性及其潜在分子机制进行了研究。戈米辛L1对A2780和SKOV3卵巢癌细胞表现出强大的细胞毒活性。流式细胞术分析显示，戈米辛L1的生长抑制作用是通过诱导细胞凋亡介导的。此外，戈米辛L1可诱导细胞内活性氧（ROS）水平升高，而抗氧化剂N-乙酰半胱氨酸可显著消除戈米辛L1诱导的细胞死亡。此外，使用抑制剂和小干扰RNA抑制NADPH氧化酶（NOX）可减弱戈米辛L1诱导的人卵巢癌细胞死亡和ROS产生。综上所述，这些数据表明，五味子果实中的木脂素戈米辛L1通过NOX调节细胞内ROS产生，从而诱导细胞凋亡性死亡。
