Davies Douglas R, Staker Bart L, Abendroth Jan A, Edwards Thomas E, Hartley Robert, Leonard Jess, Kim Hidong, Rychel Amanda L, Hewitt Stephen N, Myler Peter J, Stewart Lance J
Seattle Structural Genomics Center for Infectious Disease (http://www.ssgcid.org), USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1044-50. doi: 10.1107/S1744309111030405. Epub 2011 Aug 13.
Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and Northern Australia. Burkholderia is responsible for melioidosis, a serious infection of the skin. The enzyme 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM) catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate, a key step in the glycolytic pathway. As such it is an extensively studied enzyme and X-ray crystal structures of PGAM enzymes from multiple species have been elucidated. Vanadate is a phosphate mimic that is a powerful tool for studying enzymatic mechanisms in phosphoryl-transfer enzymes such as phosphoglycerate mutase. However, to date no X-ray crystal structures of phosphoglycerate mutase have been solved with vanadate acting as a substrate mimic. Here, two vanadate complexes together with an ensemble of substrate and fragment-bound structures that provide a comprehensive picture of the function of the Burkholderia enzyme are reported.
类鼻疽伯克霍尔德菌是一种栖息于土壤中的细菌，在东南亚和澳大利亚北部地区流行。伯克霍尔德菌会引发类鼻疽，这是一种严重的皮肤感染病。2,3-二磷酸甘油酸依赖性磷酸甘油酸变位酶（PGAM）催化3-磷酸甘油酸和2-磷酸甘油酸的相互转化，这是糖酵解途径中的关键一步。因此，它是一种经过广泛研究的酶，多个物种的PGAM酶的X射线晶体结构已被阐明。钒酸盐是一种磷酸模拟物，是研究磷酸甘油酸变位酶等磷酸转移酶的酶促机制的有力工具。然而，迄今为止，尚未解析出以钒酸盐作为底物模拟物时磷酸甘油酸变位酶的X射线晶体结构。在此，报道了两种钒酸盐复合物以及一系列底物和片段结合结构，它们全面展现了伯克霍尔德菌酶的功能。
