Ferrell M, Abendroth J, Zhang Y, Sankaran B, Edwards T E, Staker B L, Van Voorhis W C, Stewart L J, Myler P J
Seattle Structural Genomics Center for Infectious Disease, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1113-7. doi: 10.1107/S1744309111030879. Epub 2011 Aug 16.
Giardia lamblia is an anaerobic aerotolerant eukaryotic parasite of the intestines. It is believed to have diverged early from eukarya during evolution and is thus lacking in many of the typical eukaryotic organelles and biochemical pathways. Most conspicuously, mitochondria and the associated machinery of oxidative phosphorylation are absent; instead, energy is derived from substrate-level phosphorylation. Here, the 1.75 Å resolution crystal structure of G. lamblia aldose reductase heterologously expressed in Escherichia coli is reported. As in other oxidoreductases, G. lamblia aldose reductase adopts a TIM-barrel conformation with the NADP(+)-binding site located within the eight β-strands of the interior.
蓝氏贾第鞭毛虫是一种肠道内的厌氧兼性真核寄生虫。据信它在进化过程中很早就从真核生物中分化出来，因此缺乏许多典型的真核细胞器和生化途径。最明显的是，它没有线粒体和相关的氧化磷酸化机制；相反，能量来自底物水平磷酸化。在此，报道了在大肠杆菌中异源表达的蓝氏贾第鞭毛虫醛糖还原酶的1.75 Å分辨率晶体结构。与其他氧化还原酶一样，蓝氏贾第鞭毛虫醛糖还原酶采用TIM桶状构象，NADP(+)结合位点位于内部的八条β链内。
