Yan Shu, Zheng Cui, Chen Zhi-qi, Liu Rong, Li Gui-gang, Hu Wei-kun, Pei Han, Li Bin
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jie-fang Road, Wuhan, Hubei Province 430030, China.
Exp Diabetes Res. 2012;2012:743780. doi: 10.1155/2012/743780. Epub 2011 Aug 28.
Recent reports show that ER stress plays an important role in diabetic retinopathy (DR), but ER stress is a complicated process involving a network of signaling pathways and hundreds of factors, What factors involved in DR are not yet understood. We selected 89 ER stress factors from more than 200, A rat diabetes model was established by intraperitoneal injection of streptozotocin (STZ). The expression of 89 ER stress-related factors was found in the retinas of diabetic rats, at both 1- and 3-months after development of diabetes, by quantitative real-time polymerase chain reaction arrays. There were significant changes in expression levels of 13 and 12 ER stress-related factors in the diabetic rat retinas in the first and third month after the development of diabetes, Based on the array results, homocysteine- inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1(HERP), and synoviolin(HRD1) were studied further by immunofluorescence and Western blot. Immunofluorescence and Western blot analyses showed that the expression of HERP was reduced in the retinas of diabetic rats in first and third month. The expression of Hrd1 did not change significantly in the retinas of diabetic rats in the first month but was reduced in the third month.
最近的报告显示，内质网应激在糖尿病视网膜病变（DR）中起重要作用，但内质网应激是一个复杂的过程，涉及信号通路网络和数百种因子，其中涉及DR的具体因子尚不清楚。我们从200多种因子中筛选出89种内质网应激因子。通过腹腔注射链脲佐菌素（STZ）建立大鼠糖尿病模型。利用定量实时聚合酶链反应阵列，在糖尿病大鼠视网膜中发现了89种内质网应激相关因子在糖尿病发病后1个月和3个月时的表达情况。糖尿病发病后第1个月和第3个月，糖尿病大鼠视网膜中分别有13种和12种内质网应激相关因子的表达水平发生显著变化。基于阵列结果，通过免疫荧光和蛋白质印迹法进一步研究了同型半胱氨酸诱导的内质网应激诱导泛素样结构域成员1（HERP）和滑膜素（HRD1）。免疫荧光和蛋白质印迹分析表明，糖尿病大鼠视网膜中HERP的表达在第1个月和第3个月均降低。Hrd1的表达在糖尿病大鼠视网膜第1个月时无明显变化，但在第3个月时降低。
