• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过联合评估CD127和FOXP3来定义不同癌症亚型中调节性T细胞增加的比较方法。

Comparative approach to define increased regulatory T cells in different cancer subtypes by combined assessment of CD127 and FOXP3.

作者信息

Beyer Marc, Classen Sabine, Endl Elmar, Kochanek Matthias, Weihrauch Martin R, Debey-Pascher Svenja, Knolle Percy A, Schultze Joachim L

机构信息

Laboratory for Genomics and Immunoregulation, LIMES-Institute, University of Bonn, Carl-Troll-Street 31, 53115 Bonn, Germany.

出版信息

Clin Dev Immunol. 2011;2011:734036. doi: 10.1155/2011/734036. Epub 2011 Aug 28.

DOI:10.1155/2011/734036
PMID:21904560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3166761/
Abstract

In recent years an increase of functional CD4(+)CD25(+) regulatory T cells (T(reg) cells) has been established for patients with solid tumors, acute leukemias, and lymphomas. We have reported an expanded pool of CD4(+)CD25(high) T(reg) cells in patients with chronic lymphatic leukemia (CLL), multiple myeloma (MM) as well as its premalignant precursor monoclonal gammopathy of undetermined significance (MGUS). In healthy individuals, low-level expression of CD127 on T cells in addition to the expression of FOXP3 has been associated with T(reg) cells. Here, we demonstrate that the expanded FOXP3(+) T-cell population in patients with colorectal cancer, CLL, MGUS, MM, follicular lymphoma, and Hodgkin's disease are exclusively CD127(low) T(reg) cells and were strongly suppressive. A significant portion of CD127(low)FOXP3(+) T(reg) cells expressed only low levels of CD25 suggesting that the previously reported expansion of CD25(+) T(reg) cells underestimates the true expansion. The assessment of CCR7 and CD45RA expression on the expanded CD4(+)CD127(low)FOXP3(+) T(reg) cells revealed an increase of both naïve as well as central and effector memory T(reg) cells in peripheral blood. Our data strongly support superiority of combined CD127 and FOXP3 analysis in comparison to CD25 and FOXP3 assessment for further quantification of T(reg) cells in malignant diseases.

摘要

近年来，已证实实体瘤、急性白血病和淋巴瘤患者体内功能性CD4(+)CD25(+)调节性T细胞（T(reg)细胞）数量增加。我们报道过，慢性淋巴细胞白血病（CLL）、多发性骨髓瘤（MM）及其癌前病变意义未明的单克隆丙种球蛋白病（MGUS）患者体内CD4(+)CD25(高)T(reg)细胞池有所扩大。在健康个体中，T细胞上除了表达FOXP3外，CD127低水平表达也与T(reg)细胞有关。在此，我们证明，结直肠癌、CLL、MGUS、MM、滤泡性淋巴瘤和霍奇金病患者体内扩增的FOXP3(+) T细胞群体均为CD127(低)T(reg)细胞，且具有强抑制性。相当一部分CD127(低)FOXP3(+) T(reg)细胞仅低水平表达CD25，这表明先前报道的CD25(+) T(reg)细胞扩增低估了实际的扩增情况。对扩增的CD4(+)CD127(低)FOXP3(+) T(reg)细胞上CCR7和CD45RA表达的评估显示，外周血中幼稚型以及中枢和效应记忆T(reg)细胞均有所增加。我们的数据有力地支持了与CD25和FOXP3评估相比，联合CD127和FOXP3分析在进一步定量恶性疾病中T(reg)细胞方面具有优越性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/36441afcf4ef/CDI2011-734036.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/401b86fb66ec/CDI2011-734036.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/90591cc1ea55/CDI2011-734036.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/72a3846aabd1/CDI2011-734036.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/88655135ce84/CDI2011-734036.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/36441afcf4ef/CDI2011-734036.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/401b86fb66ec/CDI2011-734036.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/90591cc1ea55/CDI2011-734036.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/72a3846aabd1/CDI2011-734036.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/88655135ce84/CDI2011-734036.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/388b/3166761/36441afcf4ef/CDI2011-734036.005.jpg

相似文献

1
Comparative approach to define increased regulatory T cells in different cancer subtypes by combined assessment of CD127 and FOXP3.通过联合评估CD127和FOXP3来定义不同癌症亚型中调节性T细胞增加的比较方法。
Clin Dev Immunol. 2011;2011:734036. doi: 10.1155/2011/734036. Epub 2011 Aug 28.
2
Interleukin-7 matures suppressive CD127(+) forkhead box P3 (FoxP3)(+) T cells into CD127(-) CD25(high) FoxP3(+) regulatory T cells.白细胞介素-7 将抑制性 CD127(+) 叉头框 P3 (FoxP3)(+) T 细胞成熟为 CD127(-) CD25(高) FoxP3(+) 调节性 T 细胞。
Clin Exp Immunol. 2011 Jul;165(1):60-76. doi: 10.1111/j.1365-2249.2011.04334.x. Epub 2011 Mar 17.
3
Reduced frequency and functional defects of CD4CD25CD127 regulatory T cells in patients with unexplained recurrent spontaneous abortion.原因不明复发性自然流产患者中 CD4+CD25+CD127-调节性 T 细胞频率降低和功能缺陷。
Reprod Biol Endocrinol. 2020 Jun 10;18(1):62. doi: 10.1186/s12958-020-00619-7.
4
CD4(+)CD25(+)CD127(-) and CD4(+)CD25(+)Foxp3(+) Regulatory T Cell Subsets in Mediating Autoimmune Reactivity in Systemic Lupus Erythematosus Patients.CD4(+)CD25(+)CD127(-)和CD4(+)CD25(+)Foxp3(+)调节性T细胞亚群在系统性红斑狼疮患者自身免疫反应中的作用
Arch Immunol Ther Exp (Warsz). 2016 Oct;64(5):399-407. doi: 10.1007/s00005-016-0399-5. Epub 2016 May 7.
5
The calcineurin inhibitor tacrolimus allows the induction of functional CD4CD25 regulatory T cells by rabbit anti-thymocyte globulins.钙调磷酸酶抑制剂他克莫司通过兔抗胸腺球蛋白诱导功能性 CD4CD25 调节性 T 细胞。
Clin Exp Immunol. 2010 Aug;161(2):364-77. doi: 10.1111/j.1365-2249.2010.04183.x. Epub 2010 May 28.
6
Human adipose-tissue derived mesenchymal stem cells induce functional de-novo regulatory T cells with methylated FOXP3 gene DNA.人脂肪组织来源的间充质干细胞诱导具有甲基化 FOXP3 基因 DNA 的功能性新的调节性 T 细胞。
Clin Exp Immunol. 2013 Aug;173(2):343-54. doi: 10.1111/cei.12120.
7
Are CD4+CD25-Foxp3+ cells in untreated new-onset lupus patients regulatory T cells?未经治疗的新发狼疮患者中的 CD4+CD25-Foxp3+细胞是否为调节性 T 细胞？
Arthritis Res Ther. 2009;11(5):R153. doi: 10.1186/ar2829. Epub 2009 Oct 12.
8
Providence of the CD25 KIR CD127 FOXP3 CD8 T-cell subset determines the dynamics of tumor immune surveillance.CD25 KIR CD127 FOXP3 CD8 T 细胞亚群的Providence 决定了肿瘤免疫监视的动态。
Immunol Cell Biol. 2018 Nov;96(10):1035-1048. doi: 10.1111/imcb.12166. Epub 2018 Aug 17.
9
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.CD127的表达与人类CD4+调节性T细胞的FoxP3及抑制功能呈负相关。
J Exp Med. 2006 Jul 10;203(7):1701-11. doi: 10.1084/jem.20060772. Epub 2006 Jul 3.
10
Lower proportions of CD4+CD25(high) and CD4+FoxP3, but not CD4+CD25+CD127(low) FoxP3+ T cell levels in children with autoimmune thyroid diseases.自身免疫性甲状腺疾病患儿 CD4+CD25(high) 和 CD4+FoxP3 的比例较低，但 CD4+CD25+CD127(low)FoxP3+T 细胞水平没有变化。
Autoimmunity. 2013 May;46(3):222-30. doi: 10.3109/08916934.2012.751981. Epub 2013 Jan 22.

引用本文的文献

1
Neutrophils unveiled in chronic lymphocytic leukemia.慢性淋巴细胞白血病中发现的中性粒细胞。
Front Immunol. 2025 Aug 20;16:1609754. doi: 10.3389/fimmu.2025.1609754. eCollection 2025.
2
Standardized astragalus extract for attenuation of the immunosuppression induced by strenuous physical exercise: randomized controlled trial.标准化黄芪提取物对剧烈运动引起的免疫抑制的衰减作用：随机对照试验。
J Int Soc Sports Nutr. 2021 Jul 16;18(1):57. doi: 10.1186/s12970-021-00425-5.
3
T Cells in Chronic Lymphocytic Leukemia: A Two-Edged Sword.慢性淋巴细胞白血病中的 T 细胞：一把双刃剑。

本文引用的文献

1
Elevated myeloid-derived suppressor cells in pancreatic, esophageal and gastric cancer are an independent prognostic factor and are associated with significant elevation of the Th2 cytokine interleukin-13.在胰腺癌、食管癌和胃癌中，髓源性抑制细胞的升高是一个独立的预后因素，并且与 Th2 细胞因子白细胞介素-13 的显著升高相关。
Cancer Immunol Immunother. 2011 Oct;60(10):1419-30. doi: 10.1007/s00262-011-1028-0. Epub 2011 Jun 5.
2
Regulatory T cells predict the time to initial treatment in early stage chronic lymphocytic leukemia.调节性 T 细胞可预测早期慢性淋巴细胞白血病的初始治疗时间。
Cancer. 2011 May 15;117(10):2163-9. doi: 10.1002/cncr.25752. Epub 2010 Nov 18.
3
Front Immunol. 2021 Jan 20;11:612244. doi: 10.3389/fimmu.2020.612244. eCollection 2020.
4
Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.用于人类调节性T细胞功能表型分析的标准化11色流式细胞术检测板
J Biol Methods. 2020 Apr 13;7(2):e131. doi: 10.14440/jbm.2020.325. eCollection 2020.
5
Immunohistochemical distribution of FOXP3+ regulatory T cells in colorectal cancer patients.结直肠癌患者中FOXP3 +调节性T细胞的免疫组化分布
Int J Clin Exp Pathol. 2018 Apr 1;11(4):1841-1854. eCollection 2018.
6
Lymphocyte Subsets and Inflammatory Cytokines of Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma.意义未明单克隆丙种球蛋白血症和多发性骨髓瘤的淋巴细胞亚群和炎症细胞因子。
Int J Mol Sci. 2019 Jun 10;20(11):2822. doi: 10.3390/ijms20112822.
7
Immunotherapeutics in Multiple Myeloma: How Can Translational Mouse Models Help?多发性骨髓瘤的免疫疗法：转化小鼠模型如何提供帮助？
J Oncol. 2019 Apr 10;2019:2186494. doi: 10.1155/2019/2186494. eCollection 2019.
8
Preliminary investigation on the abnormal mechanism of CD4FOXP3CD25 regulatory T cells in pediatric B-cell acute lymphoblastic leukemia.儿童B细胞急性淋巴细胞白血病中CD4⁺FOXP3⁺CD25⁺调节性T细胞异常机制的初步研究
Exp Ther Med. 2018 Aug;16(2):1433-1441. doi: 10.3892/etm.2018.6326. Epub 2018 Jun 19.
9
CD4 T cells react to local increase of α-synuclein in a pathology-associated variant-dependent manner and modify brain microglia in absence of brain pathology.CD4 T细胞以病理相关变体依赖的方式对α-突触核蛋白的局部增加做出反应，并在没有脑部病理的情况下改变脑小胶质细胞。
Heliyon. 2018 Feb 1;4(1):e00513. doi: 10.1016/j.heliyon.2018.e00513. eCollection 2018 Jan.
10
Folate Receptor Alpha Peptide Vaccine Generates Immunity in Breast and Ovarian Cancer Patients.叶酸受体α肽疫苗在乳腺癌和卵巢癌患者中产生免疫应答。
Clin Cancer Res. 2018 Jul 1;24(13):3014-3025. doi: 10.1158/1078-0432.CCR-17-2499. Epub 2018 Mar 15.
Plasticity of T(reg) cells: is reprogramming of T(reg) cells possible in the presence of FOXP3?
T（reg）细胞的可塑性：FOXP3 存在的情况下 T（reg）细胞的重编程是否可能？
Int Immunopharmacol. 2011 May;11(5):555-60. doi: 10.1016/j.intimp.2010.11.024. Epub 2010 Nov 27.
4
Analysis of circulating regulatory T cells in patients with metastatic prostate cancer pre- versus post-vaccination.分析转移性前列腺癌患者接种疫苗前后循环调节性 T 细胞。
Cancer Immunol Immunother. 2011 Feb;60(2):197-206. doi: 10.1007/s00262-010-0927-9. Epub 2010 Oct 26.
5
Naturally occurring CD4+ CD25+ FOXP3+ T-regulatory cells are increased in chronic myeloid leukemia patients not in complete cytogenetic remission and can be immunosuppressive.在未达到完全细胞遗传学缓解的慢性髓性白血病患者中，自然发生的 CD4+ CD25+ FOXP3+ T 调节细胞增加，并具有免疫抑制作用。
Exp Hematol. 2010 Dec;38(12):1209-18. doi: 10.1016/j.exphem.2010.09.004. Epub 2010 Sep 18.
6
Increased regulatory T-cell frequencies in patients with advanced melanoma correlate with a generally impaired T-cell responsiveness and are restored after dendritic cell-based vaccination.晚期黑色素瘤患者调节性 T 细胞频率增加与普遍受损的 T 细胞反应相关，并在基于树突状细胞的疫苗接种后得到恢复。
Exp Dermatol. 2010 Aug;19(8):e213-21. doi: 10.1111/j.1600-0625.2009.01055.x.
7
Regulatory T cells: major players in the tumor microenvironment.调节性T细胞：肿瘤微环境中的主要参与者。
Curr Pharm Des. 2009;15(16):1879-92. doi: 10.2174/138161209788453211.
8
Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor.表达FoxP3转录因子的人CD4+ T细胞的功能划分与分化动力学
Immunity. 2009 Jun 19;30(6):899-911. doi: 10.1016/j.immuni.2009.03.019. Epub 2009 May 21.
9
Loss of FOXP3 expression in natural human CD4+CD25+ regulatory T cells upon repetitive in vitro stimulation.体外反复刺激后天然人类CD4+CD25+调节性T细胞中FOXP3表达缺失
Eur J Immunol. 2009 Apr;39(4):1088-97. doi: 10.1002/eji.200838904.
10
CD4(+)CD25(+)CD127(low/-) regulatory T cells express Foxp3 and suppress effector T cell proliferation and contribute to gastric cancers progression.CD4(+)CD25(+)CD127(low/-)调节性T细胞表达Foxp3并抑制效应T细胞增殖，促进胃癌进展。
Clin Immunol. 2009 Apr;131(1):109-18. doi: 10.1016/j.clim.2008.11.010. Epub 2009 Jan 18.