Yang Yunhuang, Ramelot Theresa A, Cort John R, Wang Huang, Ciccosanti Colleen, Jiang Mei, Janjua Haleema, Acton Thomas B, Xiao Rong, Everett John K, Montelione Gaetano T, Kennedy Michael A
Department of Chemistry and Biochemistry, Miami University, 701 East High Street, Oxford, OH 45056, USA.
J Struct Funct Genomics. 2011 Sep;12(3):175-9. doi: 10.1007/s10969-011-9117-z. Epub 2011 Sep 9.
Protein domain family YabP (PF07873) is a family of small protein domains that are conserved in a wide range of bacteria and involved in spore coat assembly during the process of sporulation. The 62-residue fragment of Dsy0195 from Desulfitobacterium hafniense, which belongs to the YabP family, exists as a homodimer in solution under the conditions used for structure determination using NMR spectroscopy. The structure of the Dsy0195 homodimer contains two identical 62-residue monomeric subunits, each consisting of five anti-parallel beta strands (β1, 23-29; β2, 31-38; β3, 41-46; β4, 49-59; β5, 69-80). The tertiary structure of the Dsy0195 monomer adopts a cylindrical fold composed of two beta sheets. The two monomer subunits fold into a homodimer about a single C2 symmetry axis, with the interface composed of two anti-parallel beta strands, β1-β1' and β5b-β5b', where β5b refers to the C-terminal half of the bent β5 strand, without any domain swapping. Potential functional regions of the Dsy0195 structure were predicted based on conserved sequence analysis. The Dsy0195 structure reported here is the first representative structure from the YabP family.
蛋白质结构域家族YabP（PF07873）是一类小蛋白质结构域家族，在多种细菌中保守存在，并在芽孢形成过程中参与芽孢衣组装。来自哈氏脱硫杆菌的Dsy0195的62个残基片段属于YabP家族，在用于核磁共振光谱结构测定的条件下，其在溶液中以同二聚体形式存在。Dsy0195同二聚体的结构包含两个相同的62个残基的单体亚基，每个亚基由五条反平行β链组成（β1，23 - 29；β2，31 - 38；β3，41 - 46；β4，49 - 59；β5，69 - 80）。Dsy0195单体的三级结构采用由两个β折叠片组成的圆柱形折叠。两个单体亚基围绕单个C2对称轴折叠成同二聚体，其界面由两条反平行β链β1 - β1'和β5b - β5b'组成，其中β5b指弯曲的β5链的C端一半，没有任何结构域交换。基于保守序列分析预测了Dsy0195结构的潜在功能区域。这里报道的Dsy0195结构是YabP家族的首个代表性结构。
